Determination of the precise location and the degree of the Choroidal neovascularization (CNV) lesion is essential for diagnosation Neovascular age-related macular degeneration (AMD) and evaluation the efficacy of treatment. Noninvasive imaging techniques with specific contrast for CNV evaluation are demanded. In this paper, two noninvasive imaging techniques, namely Optical coherence tomography (OCT) and Photoacoustic microscopy (PAM), are combined to provide specific detection of CNV for their complimentary contrast mechanisms. In vivo time-serial evaluation of Laser-induced CNV in rats is present at days 1, 3, 5, 7, 14, 21 after laser photocoagulation is applied to the rat fundus. Both OCT and PAM show that the CNV increases to its maximum at day 7 and decreases at day 14. Quantification of CNV area and CNV thickness is given. The dual-modal information of CNV is consistent with the histologic evaluation by hematoxylin and eosin (H&E) staining.

中文翻译：

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使用光学相干断层扫描和光声显微镜同时在大鼠体内评估激光诱导的脉络膜新生血管

确定脉络膜新生血管（CNV）病变的精确位置和程度对于诊断新生血管性年龄相关性黄斑变性（AMD）和评估治疗效果至关重要。需要具有特定对比度的无创成像技术用于 CNV 评估。在本文中，两种非侵入性成像技术，即光学相干断层扫描 (OCT) 和光声显微镜 (PAM)，结合起来提供 CNV 的特异性检测，因为它们具有互补的对比机制。体内在对大鼠眼底应用激光光凝后的第 1、3、5、7、14、21 天，对大鼠中激光诱导的 CNV 进行时间序列评估。OCT 和 PAM 均显示 CNV 在第 7 天增加到最大值，在第 14 天减少。给出了 CNV 面积和 CNV 厚度的量化。CNV 的双模态信息与苏木精和伊红 (H&E) 染色的组织学评价一致。