当前位置: X-MOL 学术RSC Chem. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
One-pot fluorescent assay for sensitive detection of APOBEC3A activity
RSC Chemical Biology ( IF 4.2 ) Pub Date : 2021-4-30 , DOI: 10.1039/d1cb00076d
Bingyao Wang 1 , Xiong Zhang 1 , Yafen Wang 1 , Kun Chen 1 , Fang Wang 2 , Xiaocheng Weng 1 , Xiang Zhou 1
Affiliation  

We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors.

中文翻译:

用于灵敏检测 APOBEC3A 活性的一锅荧光测定法

我们报道了一种基于荧光的一锅法,结合胞嘧啶脱氨和尿嘧啶切除来定量检测 A3A 活性。A3A和USER酶处理脱氨基后,观察520 nm处的荧光开启效应,可用于评价A3A活性和筛选抑制剂。
更新日期:2021-05-10
down
wechat
bug