One-pot fluorescent assay for sensitive detection of APOBEC3A activity,RSC Chemical Biology

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One-pot fluorescent assay for sensitive detection of APOBEC3A activity
RSC Chemical Biology ( IF 4.2 ) Pub Date : 2021-4-30 , DOI: 10.1039/d1cb00076d
Bingyao Wang ₁ , Xiong Zhang ₁ , Yafen Wang ₁ , Kun Chen ₁ , Fang Wang ₂ , Xiaocheng Weng ₁ , Xiang Zhou ₁

Affiliation

We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors.

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