The global commercialization of genetically modified (GM) crops has increased the likelihood of feed-derived transgenic DNA uptake and raised concerns regarding the fate of such DNA fragments in animals consuming a GM diet. The present study applied a novel droplet digital polymerase chain reaction (ddPCR) method to quantitatively detect the concentrations of feed-derived transgenic (Cry1Ie) and endogenous (rubisco) plant DNA in the livers, intestinal tracts and muscles of zebrafish (Danio rerio) fed GM maize for uantifying feed-derived DNA in animal tissues as a more sensitive and reliable alternative to qPCR.

中文翻译：

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使用液滴数字 PCR 检测饲喂转基因玉米的斑马鱼 (Danio rerio) 组织中的植物 DNA

转基因 (GM) 作物的全球商业化增加了饲料来源的转基因 DNA 摄取的可能性，并引发了人们对食用转基因饮食的动物中此类 DNA 片段的命运的担忧。本研究采用新型液滴数字聚合酶链反应 (ddPCR) 方法定量检测饲料来源的转基因 ( Cry1Ie ) 和内源性 ( rubisco ) 植物 DNA 在斑马鱼 ( Danio rerio )肝脏、肠道和肌肉中的浓度。转基因玉米用于在动物组织中对饲料来源的 DNA 进行定量分析，作为 qPCR 的一种更灵敏、更可靠的替代方法。