Recent research has demonstrated that swine viruses can be transmitted via feed. Chemical feed additives have been suggested for the mitigation of these viruses in complete feed. Therefore, the objective of this study was to evaluate the efficacy of a commercially available formaldehyde-based feed additive, medium chain fatty acid blend (MCFA), and commercially available fatty acid-based products for mitigation of porcine epidemic diarrhea virus (PEDV) and porcine reproductive and respiratory syndrome virus (PRRSV) in a feed matrix. Treatments consisted of: 1) non-treated positive control, 2) 0.33% commercial formaldehyde-based product (Sal Curb; Kemin Industries, Inc.; Des Moines, IA), 3) 0.5% MCFA blend (1:1:1 ratio of C6:0, C8:0, and C10:0, Sigma Aldrich, St. Louis, MO), 4) 0.25%, 5) 0.5%, or 6) 1% of commercial dry mono and diglyceride-based product (Furst Strike; Furst-McNess Company, Freeport, IL), 7) 0.25%, 8) 0.5%, or 9) 1% of commercial dry mono and diglyceride-based product (Furst Protect; Furst-McNess Company, Freeport, IL), 10) 0.25%, 11) 0.5%, or 12) 1% dry mono and diglyceride-based experimental product (Furst-McNess Company, Freeport, IL) with 3 replications/treatment. Treatments were applied to complete swine feed before inoculation with 106 TCID50/g of feed with PEDV or PRRSV. Post inoculation feed was held at ambient temperature for 24 h before being analyzed via qRT-PCR. The analyzed values represent the cycle threshold. Formaldehyde and MCFA decreased (P < 0.05) the detectable RNA of PEDV and PRRSV compared to all other treatments. Furst Strike, Furst Protect, and the experimental product did not significantly impact detectability of PEDV or PRRSV RNA. In conclusion, MCFA and formaldehyde treatments are effective at reducing detection of RNA from PEDV and PRRSV in feed.

中文翻译：

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PSVI-4 中链脂肪酸和基于脂肪酸的饲料添加剂作为猪流行性腹泻病毒和猪繁殖与呼吸综合征病毒缓解策略的功效

最近的研究表明，猪病毒可以通过饲料传播。已建议使用化学饲料添加剂来减轻完全饲料中的这些病毒。因此，本研究的目的是评估市售的基于甲醛的饲料添加剂、中链脂肪酸混合物 (MCFA) 和市售的基于脂肪酸的产品对缓解猪流行性腹泻病毒 (PEDV) 和饲料基质中的猪繁殖与呼吸综合征病毒 (PRRSV)。处理包括：1) 未经处理的阳性对照，2) 0.33% 商用甲醛基产品（Sal Curb；Kemin Industries, Inc.；爱荷华州得梅因），3) 0.5% MCFA 混合物（1:1:1 比例C6:0、C8:0 和 C10:0，Sigma Aldrich, St. Louis, MO), 4) 0.25%, 5) 0.5%, 或 6) 1% 的商用干式甘油单酯和双酯基产品（Furst Strike；Furst-McNess 公司，弗里波特，伊利诺伊州），7) 0.25%，8) 0.5%，或 9) 1% 的商用干式甘油单酯和甘油二酯-基产品（Furst Protect；Furst-McNess Company，Freeport，IL），10) 0.25%，11) 0.5%，或 12) 1% 干的基于甘油单酯和二酯的实验产品（Furst-McNess Company，Freeport，IL） 3 次重复/处理。在接种 106 TCID50/g PEDV 或 PRRSV 饲料之前，对完整的猪饲料进行处理。接种后的饲料在环境温度下保持 24 小时，然后通过 qRT-PCR 进行分析。分析值代表循环阈值。与所有其他处理相比，甲醛和 MCFA 降低了（P < 0.05）PEDV 和 PRRSV 的可检测 RNA。Furst Strike, Furst Protect, 并且实验产品对 PEDV 或 PRRSV RNA 的可检测性没有显着影响。总之，MCFA 和甲醛处理可有效减少饲料中 PEDV 和 PRRSV 的 RNA 检测。