Colostrum intake by neonatal piglets can be measured using the immunoglobulin immunocrit assay (iCrit). Lactocrine effects occur when maternally derived, milk-borne bioactive factors are transferred to the neonatal circulation with consumption of colostrum during nursing and affect development of somatic tissues, which can have long-term consequences in adulthood. Lactocrine deficiency, indicated by low neonatal iCrit, altered uterine gene expression and reduced fecundity in adult, neonatally lactocrine-deficient gilts. Litter size in pigs is dependent on both ovarian and uterine function. It was hypothesized that lactocrine deficiency affects development of ovarian follicles in gilts. The objective was to determine the number of primordial, primary, and secondary follicles in ovaries of gilts with high (12% ± 0.5; n = 10) or low (1.9% ± 0.4; n = 10) iCrit, determined on postnatal day (PND) 1 after birth. Paired high- and low-iCrit gilts were chosen from the same litters (birth weight; 3.1 ± 0.2 lbs). On PND 14, ovaries were collected and histological sections prepared (3 sections per animal; 30–150 µm apart). Ovarian follicles in each section were staged and the number of follicles in each category were quantified and subjected to ANOVA. Total number of ovarian follicles did not differ with iCrit (P = 0.55; 1,370.6 ± 147.8 follicles per section). The proportion of primordial, primary, and secondary follicles was 89.6 ± 1.15%, 7.7 ± 0.87%, 2.7 ± 0.51%, respectively. The number of primordial (P = 0.55), primary (P = 0.64), and secondary (P = 0.93) follicles did not differ with iCrit. Results indicate that ovarian follicular development of neonatal gilts is not sensitive to immunocrit status. Although lactocrine deficiency did not influence the ovarian follicular profile at PND 14, it remains unknown whether lactocrine programming alters ovarian follicular dynamics in neonatally lactocrine-deficient adults. USDA is an equal opportunity provider and employer.

中文翻译：

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52 产后第 14 天高和低免疫比容后备母猪的卵巢滤泡分析

新生仔猪的初乳摄入量可以使用免疫球蛋白免疫比色法 (iCrit) 来测量。当母体来源的乳源性生物活性因子在哺乳期间随着初乳的消耗而转移到新生儿循环中并影响体细胞组织的发育时，就会发生泌乳素效应，这可能会对成年期产生长期影响。新生儿 iCrit 低表明泌乳素缺乏会改变成年、新生儿泌乳素缺乏后备母猪的子宫基因表达并降低繁殖力。猪的窝产仔数取决于卵巢和子宫功能。据推测，泌乳素缺乏会影响后备母猪卵巢卵泡的发育。目的是确定高 (12% ± 0.5; n = 10) 或低 (1.9% ± 0.4; n = 10) iCrit，在出生后第 1 天 (PND) 确定。从同一窝（出生体重；3.1 ± 0.2 磅）中选择配对的高 iCrit 和低 iCrit 后备母猪。在 PND 14 上，收集卵巢并制备组织切片（每只动物 3 个切片；相距 30–150 µm）。对每个切片中的卵巢卵泡进行分期，并对每个类别中的卵泡数量进行量化并进行方差分析。卵巢卵泡的总数与 iCrit 没有差异（P = 0.55；每节 1,370.6 ± 147.8 个卵泡）。原始、初级和次级卵泡的比例分别为89.6±1.15%、7.7±0.87%、2.7±0.51%。原始 (P = 0.55)、初级 (P = 0.64) 和次级 (P = 0.93) 卵泡的数量与 iCrit 没有差异。结果表明，新生后备母猪的卵巢卵泡发育对免疫比容状态不敏感。尽管泌乳素缺乏在 PND 14 时并未影响卵巢卵泡的分布，但尚不清楚泌乳素程序是否会改变新生儿泌乳素缺乏成人的卵巢卵泡动力学。USDA 是一个机会均等的提供者和雇主。