Although sugarcane produces a large amount of energy per hectare, its ensiled storage leads to losses, which makes it one of the most expensive options for animal feeding. These losses occur due to fermentation of sugars and organic acids by yeasts, producing alcohol and gas. Inoculants containing Lactobacillus buchneri increase acetic acid production, which has effect against yeast. In this study, we produced egg yolk antibodies (IgY) against yeasts that ferment lactate (Candida glabrata, Pichia kudriavzevii, and Pichia manshurica) and sugar (Saccharomyces cerevisiae, Torulospora delbrueckii, Schizosaccharomyces pombe and Debaryomyces etchellsii) isolated in sugarcane silage. Shortly before ensiling, doses of 0, 175, 350, and 700 g/t IgY, and Lactobacillus buchneri were applied to chopped forage. Gas losses were calculated using experimental silos in triplicate, according to the equation: G = (PCf - PCa)/(MFf) × 100, in which: G = gas losses (kg/t); PCf = weight of full silo (equipped with a Bunsen valve to allow gas outflow) at sealing (kg); PCa = weight of full bucket at opening (kg); MFf = forage mass at sealing (kg). The data were analyzed as a completely randomized design and submitted to analysis of variance and regression using the SAS (SAS, 1998), which was chosen because of the significance of the regression parameters, tested by Tukey’s test (P < 0.05) and the values of the coefficients of determination. Thirty days after sealing the L. buchneri inoculant and doses of 350 and 700 g/t IgY promoted less gas losses than control that had no any product (12.74; 15.59 and 15.28 versus 27.74, respectively, P < 0.05) and the L. buchneri did not differ from IgY doses. The gas losses decreased quadratically with the anti-yeast addition, estimating maximum of 530 g/t fresh forage. We conclude that anti-yeast use reduces gas production during fermentation. With continuity of this research, we may obtain a roughage with higher nutritional value, as its mode of action in yeast control does not involve consumption of sugars or organic acids.

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156 抗酵母对甘蔗青贮产气的影响

尽管甘蔗每公顷产生大量能量，但其青贮储存会导致损失，这使其成为动物饲养最昂贵的选择之一。这些损失是由于酵母发酵糖和有机酸，产生酒精和气体。含有布氏乳杆菌的接种剂会增加乙酸的产生，这对酵母菌有作用。在这项研究中，我们生产了针对发酵甘蔗青贮中分离的乳酸（光滑念珠菌、库氏毕赤酵母和甘蔗毕赤酵母）和糖（酿酒酵母、德氏圆孢菌、粟酒裂殖酵母和德巴利酵母）的酵母的蛋黄抗体 (IgY)。在青贮前不久，将 0、175、350 和 700 g/t IgY 和布氏乳杆菌的剂量应用于切碎的草料。使用一式三份的实验筒仓计算气体损失，根据公式：G = (PCf - PCa)/(MFf) × 100，其中： G = 气体损失 (kg/t)；PCf = 密封时满仓的重量（配备本生阀以允许气体流出）（kg）；PCa = 开启时满桶的重量（kg）；MFf = 密封时的草料质量 (kg)。将数据作为完全随机设计进行分析，并使用 SAS (SAS, 1998) 进行方差和回归分析，因为回归参数的显着性而选择该方法，通过 Tukey 检验 (P < 0.05) 和决定系数的值。密封 L. buchneri 接种剂 30 天后，350 和 700 g/t IgY 的气体损失比没有任何产品的对照组少（分别为 12.74；15.59 和 15.28 对 27.74，P < 0。05) 和 L. buchneri 与 IgY 剂量没有区别。随着抗酵母的添加，气体损失呈二次方下降，估计最大值为 530 g/t 新鲜草料。我们得出结论，抗酵母的使用减少了发酵过程中的气体产生。随着这项研究的持续进行，我们可能会获得具有更高营养价值的粗饲料，因为其在酵母控制中的作用方式不涉及糖或有机酸的消耗。