The objective of this experiment was to investigate the effects of dietary supplementation of Bacillus amyloquefaciens on total and differential blood cell count in weaned pigs experimentally infected with a pathogenic E. coli. A total of 50 weaned pigs (7.41 ± 1.35 kg) were individually housed in disease containment rooms and randomly assigned to one of the 5 treatments: sham control (CON-), sham B. amyloquefaciens (BAM-), challenged control (CON+), challenged B. amyloquefaciens (BAM+) and challenged carbadox (CAR+). The experiment lasted 28 days with 7 days’ adaptation and 21 days after the first E. coli inoculation. The doses of F18 E. coli inoculum were 1010 CFU/3 mL oral dose daily for 3 consecutive days. Whole blood samples were collected from all pigs on d -7, and d 0, 7, 14, and 21 post infection (PI) to measure total and differential blood cell count by complete blood count (CBC) analysis. Supplementation of BAM or CAR increased (P < 0.05) either the percentage or the number of lymphocytes on d 0 before E. coli inoculation. E. coli challenge increased (P < 0.05) white blood cell (WBC) count on d 7 and 21 PI, while supplementation of BAM tended (P < 0.10) to have low WBC on d 7 PI and had lower (P < 0.05) WBC on d 21 PI compared with CON+. Pigs in BAM+ also had lower (P < 0.05) neutrophil count on d 14 PI, pigs fed with CAR had lower (P < 0.05) neutrophil count on d 14 and 21 PI, compared with pigs in CON+. No difference was observed in red blood cell profile among all treatments throughout the experiment. In conclusion, pigs fed with B. amyloquefaciens have similar systemic immune response to pigs in antibiotic group and have relatively lower systemic inflammation caused by E. coli compared with control group.

中文翻译：

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PSII-14 解淀粉芽孢杆菌对实验性感染致病性大肠杆菌的断奶猪的全身免疫的补充

本实验的目的是研究膳食补充解淀粉芽孢杆菌对实验性感染致病性大肠杆菌的断奶猪的总血细胞计数和差异血细胞计数的影响。总共 50 头断奶猪 (7.41 ± 1.35 kg) 被单独饲养在疾病控制室中，并随机分配到 5 种处理中的一种：假控制 (CON-)、假解淀粉芽孢杆菌 (BAM-)、攻击控制 (CON+) ，挑战解淀粉芽孢杆菌 (BAM+) 和挑战卡巴多 (CAR+)。实验持续28天，适应7天，第一次接种大肠杆菌后21天。F18 大肠杆菌接种剂的剂量为每天 1010 CFU/3 mL 口服剂量，连续 3 天。在第 -7 天和第 0、7、14 天从所有猪身上采集全血样本，21 感染后 (PI) 通过全血细胞计数 (CBC) 分析测量总血细胞计数和分类血细胞计数。补充 BAM 或 CAR 增加了 (P < 0.05) 大肠杆菌接种前第 0 天淋巴细胞的百分比或数量。大肠杆菌攻击增加（P < 0.05）白细胞（WBC）计数在第 7 天和第 21 天，而补充 BAM 倾向于（P < 0.10）在第 7 天 PI 具有低 WBC 和更低（P < 0.10） ; 0.05) 与 CON+ 相比，第 21 天 PI 上的 WBC。与 CON+ 中的猪相比，BAM+ 中的猪在 PI 14 天的中性粒细胞计数也较低（P < 0.05），CAR 喂养的猪在 PI 14 天和 21 天的中性粒细胞计数较低（P < 0.05）。在整个实验过程中，所有处理中的红细胞谱均未观察到差异。总之，用 B 喂养的猪。