Recent adoption of post cervical artificial insemination facilitates lowered concentrations of semen inseminated. In order to achieve this reduced concentration, the volume of the insemination dose has been reduced and replaced with additional air in the semen storage container. The objective of this study was to investigate the effects of semen volume, air contact inside semen dose tubes, daily agitation of semen doses and extender type on semen quality, thermo-resistance and bacterial growth in extended boar semen doses over 7 days of liquid storage at 17 C. Ejaculates from 4 terminal cross-bred boars were collected for 4 weeks and used in the 3 x 2 x 2 factorial study. The effects of treatment (CON: 80ml doses sealed at the top of the tube; 40HIGH: 40ml doses sealed at top of tube; and 40LOW: 40ml doses sealed at top of the liquid), extender type (long-term vs short-term), and agitation (agitated vs not agitated) were investigated. The result of the study revealed that motility (P=0.014) and viability (P=0.007) in 40HIGH were lower than CON. pH (P< 0.001) was higher in 40HIGH compared to CON. Agitation did not impact motility (P=0.541), viability (P=0.406) or morphology (P=0.970) while long-term extender maintained higher motility (P=0.034), viability (P< 0.001) and normal acrosomes (P< 0.001) compared to short-term extender. VAP (P=0.039) of 40HIGH was lower than CON in a thermo-resistance test. Bacteria were cultured on both sheep’s blood and MacConkey agar and neither treatment (P=0.798; 0.766) nor agitation (P=0.396; 0.476) impacted bacterial growth in this study. In conclusion, semen doses prepared with 80mL or 40mL of total volume with minimal air contact in the tubes yield more desirable semen quality, as air contact negatively impacts boar semen pH and sperm motility. Additionally, regardless of volume or air exposure, daily agitation of boar semen doses did not affect semen quality.

中文翻译：

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PSV-8 空气暴露和搅拌对储存的公猪精液样品质量的影响

最近采用宫颈后人工授精有助于降低受精精液的浓度。为了达到这种降低的浓度，授精剂量的体积已经减少并用精液储存容器中的额外空气代替。本研究的目的是调查精液量、精液剂量管内的空气接触、精液剂量的每日搅动和稀释剂类型对液体储存 7 天的延长公猪精液剂量的精液质量、耐热性和细菌生长的影响在 17 摄氏度。收集 4 头终端杂交公猪的精液 4 周并用于 3 x 2 x 2 因子研究。治疗效果（CON：80ml 剂量密封在试管顶部；40HIGH：40ml 剂量密封在试管顶部；40LOW：40ml 剂量密封在液体顶部），研究了稀释剂类型（长期与短期）和搅拌（搅拌与不搅拌）。研究结果表明，40HIGH的运动性（P=0.014）和活力（P=0.007）低于CON。与 CON 相比，40HIGH 中的 pH (P＜0.001) 更高。搅拌不影响运动性 (P=0.541)、活力 (P=0.406) 或形态 (P=0.970)，而长期稀释剂保持更高的运动性 (P=0.034)、活力 (P<0.001) 和正常顶体 (P<0.001) 0.001) 与短期延长剂相比。在耐热试验中，40HIGH的VAP（P=0.039）低于CON。在羊血和麦康凯琼脂上均培养了细菌，在本研究中，无论是处理（P=0.798；0.766）还是搅动（P=0.396；0.476）都不会影响细菌生长。综上所述，使用 80mL 或 40mL 总体积制备的精液剂量在试管中与空气接触最少，可产生更理想的精液质量，因为空气接触会对公猪精液 pH 值和精子活力产生负面影响。此外，无论体积或空气暴露量如何，公猪精液剂量的每日搅动都不会影响精液质量。