Improvements in the post-thaw quality of frozen semen could allow increased utilization of this technology in the swine industry. The objective of this study was to investigate the effects of natural honey inclusion in semen extender and freezing media on motility, mobility, and morphology of cryopreserved boar sperm. Ejaculates from 6 terminal cross-bred boars were collected using the gloved-hand technique for 3 weeks and used in a 2 x 3 factorial study design. Following collection, semen samples were incubated overnight in dilution extender with and without natural honey (D0: Androhep Plus; D1: Androhep Plus + 0.25% honey). The following day, the semen samples were cooled to 4 C in LEY cooling extender then frozen in freezing media containing 93% cooling extender + 6% glycerol + 1% Equex-STM Paste (F1), or freezing media with natural honey replacing 50% of the glycerol (F2) or 50% of the Equex-STM paste (F3). Semen samples were frozen using a controlled-rate freezer and stored in liquid nitrogen. Two straws per treatment for each boar were thawed and semen quality assessed. The inclusion of natural honey in dilution extender had no effect on post-thaw motility (P=0.733), progressive motility (P=0.562), or other mobility parameters (0.995≤P≥0.081). However, D1 had a higher percentage of normal acrosomes (P=0.001) and morphologically normal cells (P< 0.001) resulting from lower tail abnormalities compared to D0 (P=0.006). Post-thaw motility (P< 0.001) and progressive motility (P< 0.001) were increased in F3 compared to both F2 and F1. F1 had reduced normal acrosomes (P=0.009) and morphologically normal cells (P< 0.001) resulting from higher tail abnormalities (P< 0.001). In conclusion, the inclusion of natural honey, at 0.25%, in dilution extender helps maintain normal sperm and acrosome morphology, and replacing 50% Equex-STM Paste with honey in freezing extender improves post-thaw sperm motility and progressive motility of frozen-thawed boar semen.

中文翻译：

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PSV-9 天然蜂蜜稀释剂和冷冻稀释剂对公猪冻融精液质量的影响

冷冻精液解冻后质量的改善可以提高该技术在养猪业中的应用。本研究的目的是研究在精液稀释剂和冷冻培养基中加入天然蜂蜜对冷冻保存的公猪精子的运动性、流动性和形态的影响。使用戴手套的手技术收集 6 头终端杂交公猪的精液 3 周，并用于 2 x 3 因子研究设计。收集后，将精液样品在稀释液中孵育过夜，添加和不添加天然蜂蜜（D0：Androhep Plus；D1：Androhep Plus + 0.25% 蜂蜜）。第二天，将精液样品在 LEY 冷却稀释剂中冷却至 4°C，然后在含有 93% 冷却稀释剂 + 6% 甘油 + 1% Equex-STM Paste (F1) 的冷冻介质中冷冻，或用天然蜂蜜代替 50% 的甘油 (F2) 或 50% 的 Equex-STM 糊剂 (F3) 的冷冻介质。使用可控速率冷冻机冷冻精液样品并储存在液氮中。将每头公猪每次处理的两根吸管解冻并评估精液质量。在稀释稀释剂中加入天然蜂蜜对解冻后的运动性（P=0.733）、前向运动性（P=0.562）或其他运动性参数（0.995≤P≥0.081）没有影响。然而，与 D0 (P=0.006) 相比，由于尾部异常较低，D1 具有较高百分比的正常顶体 (P=0.001) 和形态正常细胞 (P<0.001)。与 F2 和 F1 相比，F3 的解冻后运动性 (P<0.001) 和渐进性运动性 (P<0.001) 增加。F1 减少了正常顶体 (P=0.009) 和形态正常的细胞 (P<0. 001) 由较高的尾部异常引起 (P<0.001)。总之，在稀释稀释剂中加入 0.25% 的天然蜂蜜有助于维持正常的精子和顶体形态，在冷冻稀释剂中用蜂蜜代替 50% Equex-STM 糊剂可提高解冻后精子的活力和冻融后的精子活力。公猪精液。