As a greenwater technology, coculturing microalgae with shrimp can improve the rearing water microenvironment and optimize aquaculture sustainability. However, little is known about the extent to which rearing water bacterial communities can be regulated by coculturing and how it occurs. Here, using 16S rRNA gene amplicon sequencing, we investigated the assembly of the bacterial community in a shrimp rearing system cocultured with Nannochloropsis oculata. Lower nutrient concentrations were detected in the N. oculata cocultivation system. Furthermore, a significant regulatory effect of N. oculata cocultivation on the bacterial community structure was also observed. Specifically, N. oculata cocultivation enriched the bacteria affiliated with the orders Flavobacteriales, Desulfuromonadales, Rhizobiales and Rhodobacterales but depleted some opportunistic pathogens such as Vibrionales, Sphingobacteriales and Xanthomonadales. Estimation of the β-nearest taxon index (βNIT) indicated that N. oculata cocultivation could drive the bacterial community assembly from stochastic processes to deterministic processes, and these deterministic effects were attributed to the concentrations of orthophosphate and ammonium (explained 24% of community variation) and the microalgae (explained of 12% community variation) through canonical correlation analysis (CCA) and variance partitioning analysis (VPA). Additionally, network analysis revealed that N. oculata cocultivation could also remodel the co-occurrence patterns and niche characteristics in bacterial community. By linking the modules with nutrient factors, two bacteria affiliated with the genus Psychroserpens and the and order Rhizobiales were negatively correlated with nitrate and orthophosphate, thereby identified as functional bacteria that might cooperate with N. oculata to remove nutrients. Our findings about the assembly processes and co-occurrence patterns of rearing water bacterial community will contribute to understanding and developing greenwater technology for improving rearing water quality.

作为一种绿水技术，将微藻类与虾共培养可以改善饲养水的微环境并优化水产养殖的可持续性。然而，人们对共培养对养殖细菌群落的调控程度及其发生方式知之甚少。在这里，我们使用16S rRNA基因扩增子测序，研究了与Nannochloropsis oculata共培养的虾养殖系统中细菌群落的装配。在N. oculata共培养系统中检测到较低的养分浓度。此外，还观察到了双眼猪笼草共培养对细菌群落结构的显着调节作用。具体而言，是烟草共培养使黄细菌，脱硫门菌，根瘤菌和红细菌菌相关的细菌富集，但耗尽了一些机会性病原体，如弧菌，鞘氨醇杆菌和黄单胞菌。β-最近分类指数（βNIT）的估计表明，双眼猪笼草共培养可将细菌群落组装从随机过程转变为确定性过程，这些确定性作用归因于正磷酸盐和铵盐的浓度（占群落变异的24％） ）和微藻（通过规范相关分析（CCA）和方差分区分析（VPA）解释了12％的群落变化）。此外，网络分析显示该种猪笼草共培养还可以重塑细菌群落中的共生模式和生态位特征。通过将模块与营养因子联系起来，与Psychroserpens属和根瘤菌和根瘤菌属相关的两种细菌与硝酸盐和正磷酸盐负相关，从而被​​确定为可能与眼球菌合作去除营养的功能性细菌。我们关于饲养水细菌群落的组装过程和共现模式的发现将有助于理解和开发用于改善饲养水质量的绿水技术。