A pH-responsive drug delivery system composed of N-(propyl)aniline (N-ANI) modified mesoporous silica nanoparticles as a carrier, and β-cyclodextrin (β-CD) as a cap was prepared and investigated. The system was studied for co-adsorption and co-delivery of two different drugs; an anticancer drug 5-fluorouracil (5-FU), and anti-inflammatory agent naproxen (NAP). Detailed adsorption study of the drugs themselves as well as in their mutual mixture was performed. The drug release experiments were realized in simulated body fluid (pH = 7.4) and slightly acidic medium (pH = 5) imitating the pH of tumor affected area. The experiments showed, that at physiological pH 7.4, the prepared drug delivery system released no drug, at slightly acidic medium (pH = 5) the β-CD caps were loosened, and drugs were released (88.5% and 98.7% for 5-FU and NAP, respectively). To investigate the efficiency of the designed system for the delivery in biological environment, the biocompatibility and cytotoxicity experiments were performed using U87 MG and SKBR3 cancer cells by fluorescence microscopy, flow-cytometry, MTT. apoptotic and CAM assays.

制备并研究了由N-（丙基）苯胺（N-ANI）改性的介孔二氧化硅纳米粒子为载体和β-环糊精（β-CD）为帽的pH响应药物递送系统。研究了该系统对两种不同药物的共吸附和共递送的作用。抗癌药5-氟尿嘧啶（5-FU）和抗炎药萘普生（NAP）。对药物本身及其相互混合进行了详细的吸附研究。药物释放实验是在模拟体液（pH = 7.4）和微酸性介质（pH = 5）中模拟肿瘤受影响区域的pH值进行的。实验表明，在生理pH值为7.4时，制备的药物递送系统没有释放药物，在弱酸性介质（pH = 5）下，β-CD帽松开，药物释放了（5-FU的分别为88.5％和98.7％）和NAP，分别）。为了研究所设计的系统在生物环境中递送的效率，通过荧光显微镜，流式细胞术，MTT，使用U87 MG和SKBR3癌细胞进行了生物相容性和细胞毒性实验。凋亡和CAM分析。