ABSTRACT

Chemiluminescence (CL) method, combined with the flow-injection (FI) technique, is an attractive alternative for pharmaceutical analysis. In this paper, a CL reaction between diperiodatoargentate(III) (DPA) and mitoxantrone (MTX) was observed. A strong CL signal was generated during the reaction of mitoxantrone with DPA in an alkaline medium. Thus a highly sensitive flow injection CL method was developed for the determination of mitoxantrone. Optimized experimental conditions were investigated in detail. Under the optimum conditions, the relative CL intensity was linear with the mitoxantrone concentration in the range of 8.0 × 10⁻¹⁰–5.0 × 10⁻⁷g/mL with a correlation coefficient of 0.9994 and the detection limit was 1.9 × 10⁻¹⁰g/mL. The proposed method had good reproducibility with a relative standard deviation of 1.5% (n = 11) for 1.0 × 10⁻⁷g/mL mitoxantrone. The highly sensitive method was successfully applied to the determination of mitoxantrone in pharmaceutical preparation and serum samples with satisfactory results. The possible CL reaction mechanism was also discussed briefly.

摘要

化学发光（CL）方法与流动注射（FI）技术相结合，是用于药物分析的一种有吸引力的替代方法。在本文中，观察到了二金刚烷酸酯（III）（DPA）和米托蒽醌（MTX）之间的CL反应。米托蒽醌与DPA在碱性介质中反应过程中产生了很强的CL信号。因此，开发了一种高灵敏的流动注射CL方法，用于测定米托蒽醌。优化的实验条件进行了详细研究。在最佳条件下，相对CL强度与米托蒽醌浓度在8.0×10 ^-10 –5.0×10 ^-7 g / mL范围内呈线性关系，相关系数为0.9994，检出限为1.9×10 ^-10克/毫升。拟议的方法具有良好的重现性， 对于1.0×10 ^-7 g / mL米托蒽醌，相对标准偏差为1.5％（n = 11）。该高灵敏度方法已成功用于药物制剂和血清样品中米托蒽醌的测定，结果令人满意。还简要讨论了可能的CL反应机理。