Heat shock proteins (HSPs) play essential roles in stress tolerance in insects, such as the cigarette beetle, Lasioderma serricorne, which is an important pest of stored products. However, the function of HSPs in environmental stress response in L. serricorne is poorly understood. In this study, six cDNAs encoding putative HSP70s were identified in L. serricorne (LsHSP70-1 to LsHSP70-6). The LsHSP70 proteins identified have signature motifs of insect HSP70s and exhibit high amino-acid identity with their respective orthologs from other insect species. Phylogenetic analysis showed that these LsHSP70s fall into three major clans: HSP70 in the cytosol (four genes), endoplasmic reticulum (one gene), and mitochondria (one gene). LsHSP70 genes are differentially expressed in different developmental stages. LsHSP70-4 and LsHSP70-5 are mainly expressed at the larval stage, while LsHSP70-3 shows the highest level at the pupal stage. The other genes are ubiquitously expressed. Furthermore, the expression levels of LsHSP70-1 and LsHSP70-4 are significantly upregulated upon exposure to temperatures of both 0 and 15 °C, while LsHSP70-3 is inducible at 15 °C and LsHSP70-6 at 0 °C. In addition, treatment at 0 °C causes significant downregulation of LsHSP70-2 and LsHSP70-3. Exposure to a temperature of 30 °C upregulates LsHSP70-1 expression, while 35 and 40 °C treatments result in significantly enhanced transcription of all the LsHSP70 genes. To the best of the authors’ knowledge, this is the first report on the sequence characteristics, phylogenetic relationships, and expression profiles of HSP70 genes in L. serricorne. The cold- and heat-inducible regulation of LsHSP70s suggests that these genes are related to tolerance of abnormal temperatures.

热休克蛋白 (HSPs) 在昆虫的胁迫耐受性中起着至关重要的作用，例如烟草甲虫Lasioderma serricorne，它是一种重要的储藏产品害虫。然而，HSPs 在L. serricorne环境应激反应中的功能知之甚少。在这项研究中，在L. serricorne ( LsHSP70-1到LsHSP70-6）。鉴定出的 LsHSP70 蛋白具有昆虫 HSP70 的特征基序，并与来自其他昆虫物种的各自直系同源物表现出高度的氨基酸同一性。系统发育分析表明，这些 LsHSP70 分为三个主要家族：细胞质中的 HSP70（四个基因）、内质网（一个基因）和线粒体（一个基因）。LsHSP70基因在不同发育阶段差异表达。LsHSP70-4和LsHSP70-5主要在幼虫期表达，而LsHSP70-3在蛹期表达水平最高。其他基因普遍表达。此外，LsHSP70-1和LsHSP70-4的表达水平暴露于 0 和 15°C 的温度时显着上调，而LsHSP70-3在 15°C 和LsHSP70-6在 0°C可诱导。此外，0°C 处理会导致LsHSP70-2和LsHSP70-3 的显着下调。暴露于 30 °C 的温度会上调LsHSP70-1 的表达，而 35 °C 和 40 °C 的处理会导致所有LsHSP70基因的转录显着增强。据作者所知，这是关于L. serricorne中HSP70基因的序列特征、系统发育关系和表达谱的第一份报告。冷热诱导调节LsHSP70 s 表明这些基因与异常温度的耐受性有关。