A combined approach to characterize ligand-induced solid–solid phase transitions in biomacromolecular crystals,Journal of Applied Crystallography

当前位置： X-MOL 学术 › J. Appl. Crystallogr. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

A combined approach to characterize ligand-induced solid–solid phase transitions in biomacromolecular crystals
Journal of Applied Crystallography ( IF 5.2 ) Pub Date : 2021-05-09 , DOI: 10.1107/s1600576721003137
Saminathan Ramakrishnan ₁ , Jason R Stagno ₁ , Valentin Magidson ₂ , William F Heinz ₂ , Yun-Xing Wang ₁

Affiliation

Solid–solid phase transitions (SSPTs) are widespread naturally occurring phenomena. Understanding the molecular mechanisms and kinetics of SSPTs in various crystalline materials, however, has been challenging due to technical limitations. In particular, SSPTs in biomacromolecular crystals, which may involve large-scale changes and particularly complex sets of interactions, are largely unexplored, yet may have important implications for time-resolved crystallography and for developing synthetic biomaterials. The adenine riboswitch (riboA) is an RNA control element that uses ligand-induced conformational changes to regulate gene expression. Crystals of riboA, upon the addition of a ligand, undergo an SSPT from monoclinic to triclinic to orthorhombic. Here, solution atomic force microscopy (AFM) and polarized video microscopy (PVM) are used to characterize the multiple transition states throughout the SSPT in both the forward and the reverse directions. This contribution describes detailed protocols for growing crystals directly on mica or glass surfaces for AFM and PVM characterization, respectively, as well as methods for image processing and phase-transition kinetics analysis.

中文翻译：

表征生物大分子晶体中配体诱导的固-固相变的组合方法

固-固相变（SSPT）是广泛存在的自然现象。然而，由于技术限制，了解各种晶体材料中 SSPT 的分子机制和动力学一直具有挑战性。特别是，生物大分子晶体中的 SSPT 可能涉及大规模变化和特别复杂的相互作用，在很大程度上尚未被探索，但可能对时间分辨晶体学和开发合成生物材料具有重要意义。腺嘌呤核糖开关 (riboA) 是一种 RNA 控制元件，利用配体诱导的构象变化来调节基因表达。添加配体后，riboA 晶体会经历从单斜晶系到三斜晶系再到斜方晶系的 SSPT。在这里，溶液原子力显微镜 (AFM) 和偏振视频显微镜 (PVM) 用于表征整个 SSPT 正向和反向的多个过渡态。该贡献描述了直接在云母或玻璃表面上生长晶体以分别进行 AFM 和 PVM 表征的详细协议，以及图像处理和相变动力学分析的方法。

更新日期：2021-06-03

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11