The South American tomato pinworm, Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae), is heavily targeted by insecticide applications. Methoxyfenozide is highly effective against T. absoluta with reduced side effects to natural enemies. This work aimed to (1) genetically and biochemically characterize resistance of the T. absoluta GBN population to methoxyfenozide, (2) establish cross resistance profiles with other insecticide groups and (3) monitor resistance in populations with the goal of improving T. absoluta insecticide resistance management (IRM). Methoxyfenozide resistance was completely recessive, polyfactorial and autosomal. Effective dominance revealed that 10 mg methoxyfenozide/L would be enough to eliminate susceptible homozygotes and heterozygotes, thus used to diagnose resistance in field populations. The synergism of methoxyfenozide toxicity in the resistant population for PBO (SR = 95×), DEF (SR = 51×) and DEM (SR = 45×), suggested monooxygenases, esterases and glutathione S-transferases as resistance mechanisms. However, only monooxygenase activity appeared to be involved in methoxyfenozide resistance. Resistance ratio for methoxyfenozide (2352-fold) after selection and cross-resistance ratios of a lab-selected GBN strain (“GBN-Sel”) were significant relative to a susceptible strain “JDR1-Sus” for tebufenozide (656-fold), cartap hydrochloride (10.68-fold), deltamethrin (4.70-fold), abamectin (2.65-fold), lufenuron (2.22-fold) and indoxacarb (1.92-fold), with negative cross-resistance to spinetoram (0.32-fold). Evidence of control failures was observed in 10 field populations of T. absoluta (mortalities between 13 and 76%), and all populations showed frequencies of resistant phenotypes (percentage survivorship ranging between 4 and 96%). A rational basis for managing resistance to bisacylhydrazines is discussed, along with details of recommended T. absoluta resistance management tactics.

南美番茄pin虫Tuta absoluta（Meyrick，1917年）（鳞翅目：Gelechiidae）是杀虫剂应用的主要目标。甲氧虫酰肼是针对高效T. absoluta以减少副作用的天敌。这项工作旨在（1）在遗传和生物化学上表征绝对黑麦草GBN种群对甲氧基苯脲类的抗性；（2）与其他杀虫剂组建立交叉抗药性；以及（3）监测种群中的抗药性，目的是改善绝对黑麦草。杀虫剂抗性管理（IRM）。甲氧芬尼特的耐药性是完全隐性的，多因素的和常染色体的。有效支配地位表明，10 mg甲氧基苯甲酰胺/ L足以消除易感的纯合子和杂合子，因此可用于诊断田间种群的抗药性。PBO（SR = 95×），DEF（SR = 51×）和DEM（SR = 45×），建议的单加氧酶，酯酶和谷胱甘肽S的抗性人群中甲氧基苯甲酰胺毒性的协同作用-转移酶作为抗性机制。然而，只有单加氧酶活性似乎参与了甲氧苯并噻唑的抗性。相对于易感菌株“ JDR1-Sus”对戊丁苯胺（656倍）而言，选择后的甲氧基苯甲酰胺的抗性比（2352倍）和实验室选择的GBN菌株（“ GBN-Sel”）的交叉耐药比显着，盐酸迦达普（10.68倍），溴氰菊酯（4.70倍），阿维菌素（2.65倍），氟苯磺隆（2.22倍）和茚虫威（1.92倍），对斯吡托兰具有负交叉耐药性（0.32倍）。在十个绝对种田鼠中观察到控制失败的证据。（死亡率介于13％至76％之间），并且所有人群均显示出抗性表型的频率（存活率百分比介于4％至96％之间）。用于管理bisacylhydrazines阻力合理的基础进行了讨论，有建议的细节一起T. absoluta性管理策略。