Abstract

The purpose of this study is to develop microspheres (core/shell) based on natural polymers used for bone tissue engineering. The morphology, structure, particle size, drug loading and encapsulation efficiency of the microspheres were characterized by field emission scanning electron microscope (FESEM), Fourier transform infrared spectroscopy (FTIR), ultraviolet spectrophotometer (UV) and laser particle size analyzer. The results showed that the gelatin (shell) microspheres loaded with steroidal saponins (core) prepared under the best process conditions were smooth and round, the particle size was about 4 μm. The drug loading and encapsulation efficiency were 24.7% and 97.5%, respectively. In the process of in vitro simulated release, the microspheres conformed to the first-order equation release kinetic model, and cumulative drug release in 8 days reached 85.6%. This may suggest the potential use of microspheres for treatment of bone tissue engineering over long periods.

摘要

本研究的目的是开发基于用于骨组织工程的天然聚合物的微球（核/壳）。采用场发射扫描电子显微镜(FESEM)、傅里叶变换红外光谱(FTIR)、紫外分光光度计(UV)和激光粒度分析仪对微球的形貌、结构、粒径、载药量和包封率进行表征。结果表明，在最佳工艺条件下制备的载有甾体皂苷（核）的明胶（壳）微球光滑圆润，粒径约为4 μm。载药量和包封率分别为24.7%和97.5%。在体外过程中模拟释放，微球符合一阶方程释放动力学模型，8天累积释药率达到85.6%。这可能表明微球在长期治疗骨组织工程中的潜在用途。