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The Use of Frozen, Food-Grade Blood to Successfully Maintain Colonies of Four Species of Mosquitoes (Diptera: Culicidae)
Journal of Insect Science ( IF 2.1 ) Pub Date : 2021-04-03 , DOI: 10.1093/jisesa/ieab026
Kara Tyler-Julian 1 , Constance Darrisaw 1 , Aaron Lloyd 1 , David Hoel 1
Affiliation  

An essential component of all mosquito-rearing activities is the act of blood-feeding the mosquitoes (Diptera: Culicidae). Many options exist for this purpose including live host animals and a diverse array of artificial-feeding methods. Most of the published artificial-feeding methods involve expensive materials, custom-built devices, or are labor-intensive. All of the previously published methods utilize blood sources, which are either expensive, or difficult to obtain. Additionally, much of the research into artificial blood-feeding methods for mosquitoes has focused on two species: Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse). This article presents a modified artificial blood-feeding method that uses affordable and easily sourced materials, does not require any technical knowledge to assemble, and requires minimal time and effort. The combination of inexpensive aluminum plates, Parafilm and polytetrafluoroethylene tape membranes, an electric germination mat, and frozen, food-grade blood produces exceptional feeding rates and abundant egg production. The method has been used for 2 yr at the Lee County Mosquito Control District to successfully maintain laboratory colonies of four species of mosquito: Ae. aegypti, Ae. albopictus, Aedes taeniorhynchus (Wiedemann), and Culex quinquefasciatus (Say). Variations of this method are reported, which can be used for wild and laboratory colonies of multiple species. This modified method is highly accessible for any small-scale mosquito rearing facility with labor or budgetary constraints.

中文翻译:

使用冷冻食品级血液成功维持四种蚊子(双翅目:Culicidae)的群落

所有蚊子饲养活动的一个重要组成部分是为蚊子喂血(双翅目:Culicidae)。为此目的存在许多选择,包括活的宿主动物和各种人工喂养方法。大多数已发表的人工喂养方法都涉及昂贵的材料、定制的设备,或者是劳动密集型的。所有先前公布的方法都使用血源,这些血源要么昂贵,要么难以获得。此外,针对蚊子的人工采血方法的大部分研究都集中在两个物种上:埃及伊蚊 (Linnaeus) 和白纹伊蚊 (Skuse)。本文介绍了一种改进的人工采血方法,该方法使用价格合理且易于采购的材料,不需要任何技术知识即可组装,并且需要最少的时间和精力。廉价的铝板、封口膜和聚四氟乙烯胶带膜、电发芽垫和冷冻的食品级血液的组合产生了出色的喂食率和丰富的产蛋量。该方法已在李县蚊虫控制区使用了 2 年,成功维持了四种蚊子的实验室菌落:Ae。埃及,Ae。albopictus、Aedes taeniorhynchus (Wiedemann) 和 Culex quinquefasciatus (Say)。报告了这种方法的变体,可用于多种物种的野生和实验室菌落。这种修改后的方法对于任何有劳动力或预算限制的小型蚊子饲养设施都很容易获得。食品级血液可产生出色的采食率和丰富的产蛋量。该方法已在李县蚊虫控制区使用了 2 年,成功维持了四种蚊子的实验室菌落:Ae。埃及,Ae。albopictus、Aedes taeniorhynchus (Wiedemann) 和 Culex quinquefasciatus (Say)。报告了这种方法的变体,可用于多种物种的野生和实验室菌落。这种修改后的方法对于任何有劳动力或预算限制的小型蚊子饲养设施都很容易获得。食品级血液可产生出色的采食率和丰富的产蛋量。该方法已在李县蚊虫控制区使用了 2 年,成功维持了四种蚊子的实验室菌落:Ae。埃及,Ae。albopictus、Aedes taeniorhynchus (Wiedemann) 和 Culex quinquefasciatus (Say)。报告了这种方法的变体,可用于多种物种的野生和实验室菌落。这种修改后的方法对于任何有劳动力或预算限制的小型蚊子饲养设施都很容易获得。和 Culex quinquefasciatus (说)。报告了这种方法的变体,可用于多种物种的野生和实验室菌落。这种修改后的方法对于任何有劳动力或预算限制的小型蚊子饲养设施都很容易获得。和 Culex quinquefasciatus (说)。报告了这种方法的变体,可用于多种物种的野生和实验室菌落。这种修改后的方法对于任何有劳动力或预算限制的小型蚊子饲养设施都很容易获得。
更新日期:2021-04-03
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