Background

Ulcerative colitis (UC) is an inflammatory disease of the intestine. The genetics factors play an important role in the pathogenesis of UC. SPARC exacerbates colonic inflammatory symptoms in dextran sodium sulphate-induced murine colitis. The aim of the study was to measure the gene expression and intestinal production of SPARC in patients with UC and controls as well as, to determine its correlation with histological activity.

Methods

We included 40 patients with confirmed diagnosis of UC, and 20 controls without endoscopic evidence of any type of colitis or neoplasia. The relative quantification of the gene expression was performed by real time PCR. GAPDH was used as housekeeping gene for normalization purposes and quality controls. Protein expression was determined by immunohistochemistry.

Results

The gene expression of SPARC was increased in patients with active UC vs in remission UC and vs. controls (P = 0.005). There was no significant difference between patients with remission UC and controls. The overexpression of SPARC in patients with active UC correlated significantly with mild histological activity (P = 0.06, OR = 7.77, IC = 0.77–77.9) moderate (P = 0.06, OR = 8.1, IC 95%=0.79–82.73), and severe (P = 0.03, OR = 6.5, IC 95%=1.09–38.6). Double positive SPARC+/CD16+ cells were localized mainly in submucosa, muscular layer, and adventitia, and in perivascular inflammatory infiltrates in patients with active UC.

Conclusion

The gene and protein expression of SPARC is increased in active UC. SPARC could be a marker of intestinal inflammation and its expression correlates with histological activity.

中文翻译：

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溃疡性结肠炎患者肠道分泌酸性和富含半胱氨酸蛋白 (SPARC)

背景

溃疡性结肠炎 (UC) 是一种肠道炎症性疾病。遗传因素在UC的发病机制中起重要作用。SPARC 加剧了葡聚糖硫酸钠诱导的小鼠结肠炎的结肠炎症症状。该研究的目的是测量 UC 患者和对照组中 SPARC 的基因表达和肠道产生，并确定其与组织学活动的相关性。

方法

我们纳入了 40 名确诊为 UC 的患者和 20 名没有任何类型结肠炎或肿瘤的内镜证据的对照。通过实时PCR进行基因表达的相对定量。GAPDH 用作管家基因用于标准化目的和质量控制。通过免疫组织化学测定蛋白质表达。

结果

SPARC 的基因表达在活动性 UC 患者与缓解 UC 和与对照组相比增加（P = 0.005）。缓解 UC 患者和对照组之间没有显着差异。SPARC 在活动性 UC 患者中的过表达与轻度组织学活动显着相关（P = 0.06，OR = 7.77，IC = 0.77-77.9）中度（P = 0.06，OR = 8.1，IC 95%=0.79-82.73），和严重（P = 0.03，OR = 6.5，IC 95%=1.09–38.6）。双阳性 SPARC+/CD16+ 细胞主要位于黏膜下层、肌层和外膜，以及活动性 UC 患者的血管周围炎性浸润中。

结论

SPARC 的基因和蛋白质表达在活动性 UC 中增加。SPARC 可能是肠道炎症的标志物，其表达与组织学活性相关。