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Dietary n-3 long chain polyunsaturated fatty acids affect the serum biochemical parameters, lipid-metabolism-related of gene expression and intestinal health of juvenile hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatu)
Aquaculture Nutrition ( IF 3.0 ) Pub Date : 2021-05-08 , DOI: 10.1111/anu.13276
Wenqiang An 1, 2 , Xiaohui Dong 1, 2 , Beiping Tan 1, 2 , Qihui Yang 1, 2 , Shuyan Chi 1, 2 , Shuang Zhang 1, 2 , Hongyu Liu 1, 2 , Yuanzhi Yang 1, 2
Affiliation  

To determine the effects of dietary n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFA) on lipid metabolism and intestinal health of juvenile hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatu), an 8-week feeding experiment was carried out using six isoproteic and isolipidic formula diets containing 0.65% (control), 1.00%, 1.35%, 1.70%, 2.05% and 2.40% n-3 LC-PUFA (dry matter). The results showed that the serum triglyceride and cholesterol reached the minimum in the 1.70% group and 1.35% group, respectively. The serum glucose content of the control group was significantly lower than that of the other groups (< 0.05). Compared with the control group, the expression of hepatic cpt-1 gene in the 2.05% group and atgl, apob-100 genes in the 2.40% group reached the maximum and increased significantly, while fas, accα and pparγ genes in the 2.05% and 2.40% groups decreased significantly (< 0.05). The cpt-1, atgl and pparα gene expression in the muscle increased first and then decreased, reaching the maximum in the 1.70% and 1.35% groups, while the expression of fas and pparγ significantly decreased (< 0.05), reaching the minimum in the 2.40% and 2.05% groups, respectively. Intestinal lipase and trypsin activities in the 2.05% group reached the maximum. In the intestine microbiota, Proteobacteria, Bacteroidetes and Firmicutes were the dominant phyla. The contents of Bacteroidales_S24-7_group and Lachnospiraceae increased with the addition n-3 LC-PUFA. In summary, the moderate dietary n-3 LC-PUFA (1.35%–1.70%) could up-regulate lipid catabolism gene expression and down-regulate lipid anabolism gene expression; dietary n-3 LC-PUFA significantly affected the structural integrity of the proximal intestine and improved the abundance of intestinal probiotics in hybrid grouper.

中文翻译:

日粮n-3长链多不饱和脂肪酸对杂种石斑鱼(♀Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatu)血清生化参数、基因表达的脂质代谢相关及肠道健康的影响

为了确定日粮n-3长链多不饱和脂肪酸(n-3 LC-PUFA)对杂种石斑鱼(♀Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatu)脂质代谢和肠道健康的影响,进行了8周的饲养实验使用含有 0.65%(对照)、1.00%、1.35%、1.70%、2.05% 和 2.40% n-3 LC-PUFA(干物质)的六种等蛋白和等脂配方日粮。结果显示,血清甘油三酯和胆固醇分别在1.70%组和1.35%组达到最低。对照组血清葡萄糖含量显着低于其他组( < 0.05)。与对照组相比,肝脏cpt - 1的表达2.05%组和atglapob - 100基因在2.40%组达到最大值并显着增加,而fasaccαpparγ基因在2.05%和2.40%组显着减少( <0.05)。的CPT - 1ATGLPPARα基因表达在肌肉先增加后降低,在1.70%和1.35%组达到最大,而表达FASPPARγ显著降低(< 0.05),分别达到 2.40% 和 2.05% 组的最小值。2.05%组肠道脂肪酶和胰蛋白酶活性达到最大值。在肠道微生物群中,变形菌门、拟杆菌门和厚壁菌门是优势门。Bacteroidales_S24-7_group 和 Lachnospiraceae 的含量随着 n-3 LC-PUFA 的加入而增加。综上所述,适度膳食n-3 LC-PUFA(1.35%~1.70%)可上调脂质分解代谢基因表达,下调脂质合成代谢基因表达;日粮 n-3 LC-PUFA 显着影响了近端肠道的结构完整性,并提高了杂种石斑鱼肠道益生菌的丰度。
更新日期:2021-05-08
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