The origins and taxonomy of the introduced vervet monkey population in Dania Beach, Florida has been unconfirmed due to a lack of documentation and genetic research. Our goal was to determine the introduction history, species identification, and geographic origins of the monkeys. Through interviews, historical archives, and popular media, we traced the monkeys to an escape from the Dania Chimpanzee Farm in 1948. The facility imported primates from Africa for medical research purposes. Historical archives suggest the monkeys were caught in Sierra Leone. We tested the hypothesis of West African origins using three genetic markers: one mitochondrial DNA gene (cytochrome b) and two fragments from the Y-chromosome, the sex-determining gene and the zinc-finger gene. We ran Bayesian and maximum-likelihood analyses to reconstruct phylogenetic trees. Results from all loci confirmed the species identification is Chlorocebus sabaeus. We found no variation among the sampled individuals and found the cytochrome b haplotype to be a complete match to a C. sabaeus sample from Senegal. Phylogenetic analyses showed strong support for the Dania Beach mitochondrial and Y-chromosome lineages to group within a monophyletic C. sabaeus clade endemic to West Africa. Our study provides critical baseline information to the scientific community about a little-known population of Chlorocebus monkeys that have adapted to a novel environment in the southeastern United States.

由于缺乏文献和基因研究，佛罗里达州达尼亚海滩引入的黑长尾猴种群的起源和分类尚未得到证实。我们的目标是确定猴子的引入历史、物种鉴定和地理起源。通过采访、历史档案和大众媒体，我们追踪到了 1948 年从达尼亚黑猩猩农场逃脱的猴子。该设施从非洲进口灵长类动物用于医学研究。历史档案表明，这些猴子是在塞拉利昂被捕的。我们使用三种遗传标记测试了西非起源的假设：一种线粒体 DNA 基因（细胞色素b) 和来自 Y 染色体的两个片段，性别决定基因和锌指基因。我们运行贝叶斯和最大似然分析来重建系统发育树。所有位点的结果均证实该种鉴定为Chlorocebus sabaeus。我们发现采样个体之间没有变化，并且发现细胞色素b单倍型与来自塞内加尔的C. sabaeus样本完全匹配。系统发育分析表明，强烈支持 Dania Beach 线粒体和 Y 染色体谱系在西非特有的单系C. sabaeus进化枝内分组。我们的研究为科学界提供了关于鲜为人知的绿尾藻种群的关键基线信息 适应了美国东南部新环境的猴子。