Air discharge showed significant inhibition on mycelial growth and spore germination of Fusarium oxysporum, one of the main spoilage fungi in post-harvest lotus roots which is an important economic aquatic vegetable in China. However, the antimicrobial mechanism of air discharge is not clear yet. In the present study, the effects of air discharge on F. oxysporum separated from post-harvest rotten lotus roots were characterized by analyzing surface charges, cell wall permeability, and changes in chitin and chitosan including surface morphology, functional groups, degree of deacetylation, crystallinity, and C/N ratio. After air discharge treatments, alkaline phosphatase leak assay revealed that cell wall permeability of F. oxysporum was magnified. What’s more, zeta potentials of F. oxysporum increased and negative charges on cell surfaces decreased. The ordered and compact molecular arrangements of chitin and chitosan in cell walls of F. oxysporum were reduced. The deacetylation degree of chitin and chitosan increased, and the C/N ratios of chitin and chitosan decreased. It was concluded from these results that air discharge caused the transformation in structures of chitin and chitosan, resulting in the exposure of positively charged amino groups and decrease of negative charges on cell surfaces which brought damage to the structure and function of F. oxysporum’s cell walls.

空气排放对尖孢镰刀菌的菌丝生长和孢子萌发有显着抑制作用，尖孢镰刀菌是我国重要的经济水产蔬菜莲藕收获后的主要腐败菌之一。然而，空气排放的抗菌机制尚不清楚。在本研究中，在空气排出的效果尖孢镰孢从收获后烂藕进行了表征分析表面电荷，细胞壁的通透性，以及改变甲壳质和脱乙酰壳包括表面形貌，官能团分离，脱乙酰度，结晶度和 C/N 比。空气放电处理后，碱性磷酸酶渗漏测定显示尖孢镰刀菌的细胞壁通透性被放大了。更重要的是，尖孢镰刀菌的zeta电位增加，细胞表面的负电荷减少。F. oxysporum细胞壁中几丁质和壳聚糖的有序和紧凑的分子排列减少。甲壳素和壳聚糖的脱乙酰度增加，甲壳素和壳聚糖的C/N比降低。由这些结果可以得出结论，空气放电引起了几丁质和壳聚糖的结构转变，导致带正电荷的氨基暴露，细胞表面负电荷减少，从而对尖孢镰刀菌的结构和功能造成损害。细胞壁。