The increased complexity of the healing process and interplay of various pathological factors in cutaneous wounds, in particular, chronic wounds, entails a meticulous and spatiotemporally sufficient treatment to accomplish successful therapy. Here, we have developed the hybrid bilayer scaffold system using cryogelation and electrospinning techniques to closely resemble the native three-dimensional architecture of skin. The bilayer scaffold involves curcumin dextran nanoparticles (CDNp) and cerium oxide nanoparticles (CeO₂) loaded gelatin cryogel layer and polyvinyl alcohol-poly (vinyl pyrrolidone)-iodine-potassium iodide (PPI-KI) electrospun fibrous layer towards the outer side. The developed scaffold exhibited high water uptake capability, degradation and hemocompatibility besides showing sustained and prolonged release of CDNp (65% ± 2.61 in 120 h) and iodine (66.47% ± 2.073 in 72 h). The in-vitro studies of bilayer scaffold, cryogels and electrospun sheet demonstrated enhanced cell viability and accelerated cell migration apart from displaying a strong antioxidant and antibacterial activity. The developed scaffolds elucidated a faster-wound closure rate in the in-vivo wound model in comparison to commercially available dressing and other groups. Further, in the infected wound model, the bilayer scaffold prevented wound infection and showed rapid and efficient healing of wounds. The developed dressing was observed to promote mature epithelialization with ample hair growth and increased deposition of mature collagen. Thus, the novel synergistic approach can remarkably provide new insights for the design of advanced and multifunctional wound dressing with potential application in accelerating the healing of cutaneous wounds.

愈合过程的复杂性增加以及皮肤伤口（尤其是慢性伤口）中各种病理因素的相互作用，需要进行细致且时空上充分的治疗才能成功完成治疗。在这里，我们已经开发出使用低温凝胶化和静电纺丝技术的混合双层支架系统，以非常类似于皮肤的自然三维结构。双层支架涉及姜黄素右旋糖酐纳米颗粒（CDNp）和氧化铈纳米颗粒（CeO ₂）的明胶冷冻凝胶层和聚乙烯醇-聚（乙烯基吡咯烷酮）-碘-碘化钾（PPI-KI）的电纺纤维层朝外侧。发达的支架具有高吸水能力，降解能力和血液相容性，此外还显示出CDNp（120小时内65％±2.61）和碘（72小时内66.47％±2.073）的持续和延长释放。双层脚手架，冰冻凝胶和电纺片的体外研究表明，除显示出强大的抗氧化和抗菌活性外，细胞活力增强，细胞迁移加快。与市售敷料和其他组相比，开发的支架阐明了在体内伤口模型中伤口闭合速度更快。此外，在感染的伤口模型中，双层支架可防止伤口感染并显示出快速有效的伤口愈合。观察到开发的敷料可促进成熟的上皮形成，并具有充足的毛发生长和增加的成熟胶原蛋白沉积。因此，新颖的协同方法可以显着地为先进和多功能伤口敷料的设计提供新的见识，并具有加速皮肤伤口愈合的潜在应用。