The biotechnology industry is currently seeking increased or improved enzyme efficiency and the emergence of new methods to maximize their shelf-life. Enzyme immobilization on solid supports offers characteristic features, such as recyclability, easy product recovery, improved enzyme stability, and biocatalytic properties. Biopolymers-based support materials provide cost-effective, and stability attributes to immobilized enzymes due to non-toxicity, biodegradability, biocompatibility, and the presence of multiple functional moieties. In addition, nanostructured materials with large specific surface areas and unique structural, physical, chemical, and operating characteristics have significantly contributed to biocatalytic systems for diverse biotechnological applications. Urease is a ubiquitous metalloenzyme that displays a high ability to catalyze urea's decomposition into ammonia and carbamate. This review provides information on urease immobilization using various biopolymers-based support matrices to develop nano-biocatalytic systems for biotechnological, medical, and industrial fields.

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基于脲酶的生物催化平台-经典酶的现代视角与应用前景

生物技术行业目前正在寻求提高或改善酶效率的方法，以及新方法的出现，以最大限度地延长其保质期。固定在固体支持物上的酶具有特征性，例如可回收性，易于回收的产品，改善的酶稳定性和生物催化性能。基于生物聚合物的支持材料由于无毒，可生物降解，生物相容性以及存在多个功能部分，因此可以为固定化酶提供具有成本效益的稳定性特性。另外，具有大的比表面积和独特的结构，物理，化学和操作特性的纳米结构材料极大地促进了用于多种生物技术应用的生物催化系统。脲酶是一种普遍存在的金属酶，具有很高的催化尿素分解为氨和氨基甲酸酯的能力。这篇综述提供了有关使用各种基于生物聚合物的支持基质固定尿素酶的信息，以开发用于生物技术，医学和工业领域的纳米生物催化系统。

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