Objective

The objective of this study is to prepare new dental resins with a long-lasting antimicrobial activity. Specifically, this study evaluates an approach for controlling infection in root canals using sealers containing polyhydroxyethyl methacrylate trimethylolpropane trimethacrylate (polyHEMA/TMPT) particles loaded with cetylpyridinium chloride (CPC). In addition, the physical properties of sealers containing CPC-loaded polyHEMA/TMPT particles (CLP) are determined.

Methods

PolyHEMA/TMPT particles with 10 (10%-CLP) and 25 wt.% CPC (25%-CLP) with different particle sizes were fabricated and incorporated in HEMA-based sealers. CPC-release profiles were evaluated over 14 days of immersion in water, followed by 14 days of storage and 14 days of water immersion. The antibacterial activity of these sealers against Enterococcus faecalis in dentinal tubules was assessed using a root-canal-infection model. Their sealing abilities were evaluated by fluid filtration and physical properties were tested according to the ISO 6876 standard. The long-term antibacterial activity of the cured sealer containing 25%-CLP (∼21 μm particle diameter) was re-assessed after 1 year of storage.

Results

After 28 days of immersion, 25%-CLP exhibited a higher and sustained CPC release unlike 10%-CLP. Residual bacteria in root dentinal tubules were eradicated by obturation with 25%-CLP-containing sealers. The incorporation of 25%-CLP (∼21 μm) had no adverse effects on the sealing ability and physical properties of the sealer and resulted in long-term antibacterial activity.

Significance

The incorporation of CPC-loaded particles in HEMA resins yielded endodontic sealers with long-term bactericidal activity against E. faecalis in root canals. These sealers can potentially be used to prevent recurrent apical periodontitis.

中文翻译：

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含有具有长期抗菌作用的含抗菌聚合物颗粒的牙髓封闭剂的开发

客观的

本研究的目的是制备具有持久抗菌活性的新型牙科树脂。具体而言，本研究评估了一种使用含有聚甲基丙烯酸羟乙酯三羟甲基丙烷三甲基丙烯酸酯 (polyHEMA/TMPT) 颗粒和氯化十六烷基吡啶 (CPC) 的封闭剂来控制根管感染的方法。此外，还确定了含有 CPC 负载的聚 HEMA/TMPT 颗粒 (CLP) 的密封剂的物理特性。

方法

 制造了具有不同粒径的10 (10%-CLP) 和 25 wt.% CPC (25%-CLP) 的PolyHEMA/TMPT 颗粒，并将其掺入基于 HEMA 的密封剂中。在浸入水中 14 天后评估 CPC 释放曲线，然后是 14 天的储存和 14 天的水浸。使用根管感染模型评估这些封闭剂对牙本质小管中粪肠球菌的抗菌活性。它们的密封能力通过流体过滤进行评估，并根据 ISO 6876 标准测试物理性能。在 储存 1 年后重新评估含有 25%-CLP（~21 μm 粒径）的固化封闭剂的长期抗菌活性。

结果

浸泡 28 天后，与 10%-CLP 不同，25%-CLP 表现出更高且持续的 CPC 释放。通过用含 25% CLP 的封闭剂封闭根除牙本质小管中的残留细菌。25%-CLP（~21  μm）的掺入对密封剂的密封能力和物理性能没有不利影响，并产生长期的抗菌活性。

意义

在 HEMA 树脂中加入 CPC 负载颗粒产生了对根管中的粪肠球菌具有长期杀菌活性的牙髓封闭剂。这些封闭剂可潜在地用于预防复发性根尖周炎。