Abdominal massage (AM), a traditional Chinese medicine-based treatment method, has received considerable attention in the recent years. The aim of the present study was to investigate the effect of AM on high-fat diet (HFD)-induced insulin resistance (IR) in comparison with resveratrol (RSV) treatment. Forty-eight male Sprague-Dawley rats were randomly divided into the following four groups: standard chow diet (control group), high-fat diet (model group), HFD + abdominal massage (AM group), and HFD + resveratrol (RSV group). A rat model of IR was established by feeding HFD to rats for 8 weeks followed by treatment with AM or RSV for 4 weeks. The underlying HFD-induced IR molecular mechanisms were studied in rat serum and skeletal muscles. RSV and AM significantly improved glucose intolerance, hyperglycemia, obesity, and significantly reduced lipid accumulation [triglyceride (TC), total cholesterol (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C)], adipocytokine [free fatty acids (FFA), adiponectin (ADPN)] and serum pro-inflammatory cytokines (IL-6 and TNF-α) secretion. In addition, AM activated the AMPK/SIRT1 signaling pathway in rat skeletal muscle. In conclusion, our results showed that AM could improve IR by regulating the secretion of adipocytokines, pro-inflammatory cytokines as well as related signaling pathways in the skeletal muscle of rats, which might provide insights into development of new treatment methods for the clinical treatment of IR.

腹部按摩（AM）作为一种传统的中医治疗方法，近年来受到了相当多的关注。本研究的目的是探讨与白藜芦醇 (RSV) 治疗相比，AM 对高脂饮食 (HFD) 诱导的胰岛素抵抗 (IR) 的影响。 48只雄性Sprague-Dawley大鼠随机分为4组：标准饲料组（对照组）、高脂饮食组（模型组）、HFD+腹部按摩组（AM组）、HFD+白藜芦醇组（RSV组） ）。通过给大鼠喂食HFD 8周，然后用AM或RSV治疗4周，建立IR大鼠模型。在大鼠血清和骨骼肌中研究了 HFD 诱导 IR 的潜在分子机制。 RSV和AM显着改善葡萄糖不耐症、高血糖、肥胖症，并显着降低脂质积累[甘油三酯（TC）、总胆固醇（TG）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C） ）]、脂肪细胞因子[游离脂肪酸（FFA）、脂联素（ADPN）]和血清促炎细胞因子（IL-6和TNF-α）的分泌。此外，AM 激活大鼠骨骼肌中的 AMPK/SIRT1 信号通路。总之，我们的研究结果表明，AM可以通过调节大鼠骨骼肌中脂肪细胞因子、促炎细胞因子以及相关信号通路的分泌来改善IR，这可能为临床治疗IR的新治疗方法的开发提供见解。红外。