Super-resolution microscopy (SRM) comprises a suite of techniques well-suited to probing the nanoscale landscape of genomic function and dysfunction. Offering the specificity and sensitivity that has made conventional fluorescence microscopy a cornerstone technique of biological research, SRM allows for spatial resolutions as good as 10 nanometers. Moreover, single molecule localization microscopies (SMLMs) enable examination of individual molecular targets and nanofoci allowing for the characterization of subpopulations within a single cell. This review describes how key advances in both SRM techniques and sample preparation have enabled unprecedented insights into DNA structure and function, and highlights many of these new discoveries. Ongoing development and application of these novel, highly interdisciplinary SRM assays will continue to expand the toolbox available for research into the nanoscale genomic landscape.

超分辨率显微镜 (SRM) 包括一套非常适合探测基因组功能和功能障碍的纳米级景观的技术。SRM 具有使传统荧光显微镜成为生物研究基石技术的特异性和灵敏度，可实现高达 10 纳米的空间分辨率。此外，单分子定位显微镜 (SMLM) 能够检查单个分子靶标和纳米焦点，从而可以表征单个细胞内的亚群。这篇综述描述了 SRM 技术和样品制备方面的关键进步如何实现对 DNA 结构和功能的前所未有的洞察，并重点介绍了其中的许多新发现。这些小说的不断开发和应用，