Genetic interaction (GI) analysis is a powerful genetic strategy that analyzes the fitness and phenotypes of single- and double-gene mutant cells in order to dissect the epistatic interactions between genes, categorize genes into biological pathways, and characterize genes of unknown function. GI analysis has been extensively employed in model organisms for foundational, systems-level assessment of the epistatic interactions between genes. More recently, GI analysis has been applied to microbial pathogens and has been instrumental for the study of clinically important infectious organisms. Here, we review recent advances in systems-level GI analysis of diverse microbial pathogens, including bacterial and fungal species. We focus on important applications of GI analysis across pathogens, including GI analysis as a means to decipher complex genetic networks regulating microbial virulence, antimicrobial drug resistance and host-pathogen dynamics, and GI analysis as an approach to uncover novel targets for combination antimicrobial therapeutics. Together, this review bridges our understanding of GI analysis and complex genetic networks, with applications to diverse microbial pathogens, to further our understanding of virulence, the use of antimicrobial therapeutics and host-pathogen interactions. .

中文翻译：

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微生物病原体中的遗传相互作用分析：发病机理，抗微生物药性和宿主相互作用的关系网。

遗传相互作用（GI）分析是一种强大的遗传策略，可分析单基因和双基因突变细胞的适应性和表型，以剖析基因之间的上位相互作用，将基因归类为生物途径，并鉴定功能未知的基因。GI分析已广泛用于模型生物中，用于基因之间上位相互作用的基础，系统级评估。最近，GI分析已应用于微生物病原体，并已在临床上重要的传染性生物的研究中发挥了作用。在这里，我们回顾了包括细菌和真菌在内的各种微生物病原体在系统级胃肠道分析中的最新进展。我们专注于跨病原体进行GI分析的重要应用，包括GI分析，以破译调控微生物毒力，抗微生物药物耐药性和宿主病原体动力学的复杂遗传网络，以及GI分析，以发现新型抗微生物治疗靶标的方法。总之，本综述将我们对胃肠道分析和复杂遗传网络的理解与对各种微生物病原体的应用联系起来，以加深我们对毒力，抗微生物疗法的使用以及宿主-病原体相互作用的了解。。进一步了解毒力，使用抗菌药物以及宿主与病原体之间的相互作用。。进一步了解毒力，使用抗菌药物以及宿主与病原体之间的相互作用。。