Enzymes are very important for biological processes in a living being, performing similar or multiple tasks in and out of cells, tissues and other organisms at a particular location. The abnormal activity of particular enzyme usually caused serious diseases such as Alzheimer’s disease, Parkinson’s disease, cancers, diabetes, cardiovascular diseases, arthritis etc. Hence, nondestructive and real-time visualization for certain enzyme is very important for understanding the biological issues, as well as the drug administration and drug metabolism. Fluorescent cellular probe-based enzyme detection in vitro and in vivo has become broad interest for human disease diagnostics and therapeutics. This review highlights the recent findings and designs of highly sensitive and selective fluorescent cellular probes targeting enzymes for quantitative analysis and bioimaging.

酶对于生物的生物过程非常重要，在特定位置的细胞、组织和其他生物体内外执行类似或多项任务。特定酶的异常活性通常会导致严重的疾病，如阿尔茨海默病、帕金森病、癌症、糖尿病、心血管疾病、关节炎等。因此，对某些酶进行无损和实时可视化对于理解生物学问题非常重要，以及如药物给药和药物代谢。基于荧光细胞探针的体外和体内酶检测已成为人类疾病诊断和治疗的广泛兴趣。本综述重点介绍了针对酶进行定量分析和生物成像的高灵敏度和选择性荧光细胞探针的最新发现和设计。