ABSTRACT

In the present study, we evaluated the antibacterial activity of Citrus reticulata (C. reticulata) essential oil-incorporated gelatin film solution against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The minimum inhibitory concentration (MIC) of C. reticulata essential oil (CrEO) on E. coli and S. aureus were found to be 10.1 and 9.1 mg mL⁻¹, respectively, using spectrophotometric microdilution technique. The antimicrobial effect of CrEO alone and incorporated gelatin-based film solutions (CrEO-F) against these pathogens were determined by the disc diffusion method. While the inhibition zones of CrEO were 2.4 mm and 10.05 mm, CrEO-F was reported as 1.5 and 7.8 mm against E. coli and S. aureus, respectively. These findings demonstrated that the CrEO can compete with coating agents with antimicrobial additives and it can find a place in the application areas. Besides, the chemical composition of the CrEO was determined by GC-MS.

摘要

在本研究中，我们评估了柑橘( C. reticulata ) 精油掺入的明胶薄膜溶液对大肠杆菌( E. coli ) 和金黄色葡萄球菌( S. aureus ) 的抗菌活性。使用分光光度微量稀释技术，发现网状梭菌精油 ( Cr EO) 对大肠杆菌和金黄色葡萄球菌的最低抑菌浓度 (MIC)分别为 10.1 和 9.1 mg mL ^{-1 。}Cr EO 单独和掺入明胶基薄膜溶液的抗菌作用（ CrEO-F) 对这些病原体的影响是通过圆盘扩散法测定的。CrEO 的抑菌圈分别为 2.4 毫米和 10.05 毫米，而Cr EO-F 对大肠杆菌和金黄色葡萄球菌的抑菌圈分别为 1.5 和 7.8 毫米。这些发现表明，Cr EO 可以与具有抗菌添加剂的涂层剂竞争，并且可以在应用领域找到一席之地。此外，Cr EO 的化学成分由 GC-MS 确定。