BACKGROUND Measles vaccinations have been suggested to provide immune protection and decreased measles incidence. However, there was a limited study evaluating how the measles vaccine elicits specific immune responses. OBJECTIVE This study aimed to evaluate both humoral and cellular immunity to first-dose measles vaccine Edmonston-Zagreb (EZ) in 9-month-old Indonesian infants. METHODS A cohort study was conducted on 9-month-old infants who got the first-dose of measles vaccine EZ. Measles-specific immunoglobulin G (IgG) antibody serum levels were measured using plaque-reduction microneutralization assay. Peripheral blood mononuclear cells were stimulated with a measles-specific peptide to identify a cellular immune response. Quantification of CD4+ and CD8+ T-cells producing interferon-gamma (IFN-ɣ) and interleukin 17-A (IL-17A) were conducted by flow cytometry. Humoral and cellular immune response parameters were analyzed over time. RESULTS The prevalence of seropositivity rates was 85.8% at 1-month after vaccination and 16.67% at 6-months postvaccination. Measles-specific IgG antibodies increased significantly at 1-month after measles vaccination. However, they decreased significantly 6-months after vaccination. IFN-ɣ and IL-17A secreting T-cells increased significantly at 1-month after measles vaccination. Interestingly, a significant decrease of IFN-ɣ and IL-17A secreting CD4+ T cells was noticed 6-months postvaccination compared to IFN-ɣ and IL-17A secreting CD8+ T cells. CONCLUSION Our study suggests that the first-dose measles vaccine on 9-months-old infants seems to induce both humoral and cellular immune responses that decline 6-months after vaccination.

中文翻译：

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9 个月大婴儿对首剂麻疹疫苗 Edmonston-Zagreb 的特异性抗体和细胞免疫反应分析。

背景已经建议接种麻疹疫苗以提供免疫保护和降低麻疹发病率。然而，评估麻疹疫苗如何引发特异性免疫反应的研究有限。目的 本研究旨在评估 9 个月大的印度尼西亚婴儿对首剂麻疹疫苗 Edmonston-Zagreb (EZ) 的体液和细胞免疫。方法 对接受首剂麻疹疫苗 EZ 的 9 个月大婴儿进行队列研究。麻疹特异性免疫球蛋白 G (IgG) 抗体血清水平使用减少斑块的微量中和测定法测量。用麻疹特异性肽刺激外周血单个核细胞以鉴定细胞免疫反应。通过流式细胞术对产生干扰素-γ (IFN-ɣ) 和白细胞介素 17-A (IL-17A) 的 CD4+ 和 CD8+ T 细胞进行量化。随着时间的推移分析体液和细胞免疫反应参数。结果 接种后 1 个月血清阳性率为 85.8%，接种后 6 个月为 16.67%。麻疹疫苗接种后 1 个月，麻疹特异性 IgG 抗体显着增加。然而，它们在接种疫苗后 6 个月显着下降。接种麻疹疫苗后 1 个月，分泌 IFN-ɣ 和 IL-17A 的 T 细胞显着增加。有趣的是，与分泌 IFN-ɣ 和 IL-17A 的 CD8+ T 细胞相比，在接种疫苗后 6 个月观察到分泌 IFN-ɣ 和 IL-17A 的 CD4+ T 细胞显着减少。