BACKGROUND Measles vaccinations have been suggested to provide immune protection and decreased measles incidence. However, there was a limited study evaluating how the measles vaccine elicits specific immune responses. OBJECTIVE This study aimed to evaluate both humoral and cellular immunity to first-dose measles vaccine Edmonston-Zagreb (EZ) in 9-month-old Indonesian infants. METHODS A cohort study was conducted on 9-month-old infants who got the first-dose of measles vaccine EZ. Measles-specific immunoglobulin G (IgG) antibody serum levels were measured using plaque-reduction microneutralization assay. Peripheral blood mononuclear cells were stimulated with a measles-specific peptide to identify a cellular immune response. Quantification of CD4+ and CD8+ T-cells producing interferon-gamma (IFN-ɣ) and interleukin 17-A (IL-17A) were conducted by flow cytometry. Humoral and cellular immune response parameters were analyzed over time. RESULTS The prevalence of seropositivity rates was 85.8% at 1-month after vaccination and 16.67% at 6-months postvaccination. Measles-specific IgG antibodies increased significantly at 1-month after measles vaccination. However, they decreased significantly 6-months after vaccination. IFN-ɣ and IL-17A secreting T-cells increased significantly at 1-month after measles vaccination. Interestingly, a significant decrease of IFN-ɣ and IL-17A secreting CD4+ T cells was noticed 6-months postvaccination compared to IFN-ɣ and IL-17A secreting CD8+ T cells. CONCLUSION Our study suggests that the first-dose measles vaccine on 9-months-old infants seems to induce both humoral and cellular immune responses that decline 6-months after vaccination.

中文翻译：

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分析9个月大婴儿对首剂麻疹疫苗Edmonston-Zagreb的特异性抗体和细胞免疫应答。

背景技术已建议麻疹疫苗接种可提供免疫保护并降低麻疹发病率。但是，评估麻疹疫苗如何引发特异性免疫反应的研究有限。目的本研究旨在评估印度尼西亚9个月大婴儿对第一剂麻疹疫苗埃德蒙斯顿·萨格勒布（EZ）的体液免疫和细胞免疫。方法进行了一项队列研究，研究对象是首次接种EZ麻疹疫苗的9个月大婴儿。麻疹特异性免疫球蛋白G（IgG）抗体血清水平使用噬斑减少微中和测定法进行测量。用麻疹特异性肽刺激外周血单核细胞以鉴定细胞免疫应答。通过流式细胞术对产生干扰素-γ（IFN-γ）和白介素17-A（IL-17A）的CD4 +和CD8 + T细胞进行定量。随时间分析体液和细胞免疫应答参数。结果接种疫苗后1个月的血清阳性率为85.8％，接种疫苗后6个月的血清阳性率为16.67％。麻疹疫苗接种后1个月，麻疹特异性IgG抗体显着增加。但是，接种疫苗后6个月，它们显着下降。麻疹疫苗接种后1个月，分泌IFN-γ和IL-17A的T细胞显着增加。有趣的是，与分泌IFN-γ和IL-17A的CD8 + T细胞相比，接种后6个月发现分泌IFN-γ和IL-17A的CD4 + T细胞显着降低。