To achieve a complete understanding of how organisms function, there is a need to study their fundamental unit, the cell, in its spatial context. In recent years, we have seen fast-paced technological progress to study the transcriptional content of single cells and their spatial relationships. This review highlights modern advancements in single-cell RNA-sequencing, provides an overview of the technologies that led the plant field toward spatial transcriptomics, and describes the available spatial transcriptomics approaches providing examples of their application to plant tissues. In addition, it discusses the integration of these methods to study plant tissues. Taken together, we propose a central role of spatial transcriptomics approaches in plant science.

中文翻译：

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植物科学的新时代：空间单细胞转录组学。

为了全面了解生物体的功能，有必要在其空间背景下研究它们的基本单位，即细胞。近年来，我们看到了研究单细胞转录内容及其空间关系的快节奏技术进步。这篇综述突出了单细胞 RNA 测序的现代进步，概述了引领植物领域走向空间转录组学的技术，并描述了可用的空间转录组学方法，提供了它们在植物组织中的应用实例。此外，它还讨论了这些方法的整合以研究植物组织。总之，我们提出了空间转录组学方法在植物科学中的核心作用。