Owing to their high energy density and composition, fatty acid-derived chemicals possess a wide range of applications such as biofuels, biomaterials, and other biochemical, and as a consequence, the global annual demand for products has surpassed 2 million tons. With the exhausting petroleum reservoirs and emerging environmental concerns on using petroleum feedstock, it has become indispensable to shift to a renewable-based industry. With the advancement in the field of synthetic biology and metabolic engineering, the use of microbes as factories for the production of fatty acid-derived chemicals is becoming a promising alternative approach for the production of these derivatives. Numerous metabolic approaches have been developed for conditioning the microbes to improve existing or develop new methodologies capable of efficient oleochemical production. However, there still exist several limitations that need to be addressed for the commercial viability of the microbial cell factory production. Though substantial advancement has been made toward successfully producing these fatty acids derived chemicals, a considerable amount of work needs to be done for improving the titers. In the present review, we aim to address the roadblocks impeding the heterologous production, the engineering pathway strategies implemented across the range of microbes in a detailed manner, and the commercial readiness of these molecules of immense application.

中文翻译：

---

生产脂肪醇和烷烃的微生物工程

由于其高能量密度和高成分，脂肪酸衍生化学品具有广泛的应用，如生物燃料、生物材料和其他生化产品，因此，全球对产品的年需求量已超过 200 万吨。随着石油储层的枯竭和对使用石油原料的新环境问题的关注，转向可再生能源行业已变得不可或缺。随着合成生物学和代谢工程领域的进步，使用微生物作为生产脂肪酸衍生化学品的工厂正在成为生产这些衍生物的有希望的替代方法。已经开发了许多代谢方法来调节微生物以改进现有的或开发能够有效进行油脂化学生产的新方法。然而，对于微生物细胞工厂生产的商业可行性，仍然存在一些需要解决的限制。尽管在成功生产这些脂肪酸衍生化学品方面取得了实质性进展，但需要做大量工作来提高效价。在本综述中，我们的目标是解决阻碍异源生产的障碍，详细地在微生物范围内实施的工程途径策略，以及这些具有巨大应用的分子的商业准备。对于微生物细胞工厂生产的商业可行性，仍然存在一些需要解决的限制。尽管在成功生产这些脂肪酸衍生化学品方面取得了实质性进展，但需要做大量工作来提高效价。在本综述中，我们的目标是解决阻碍异源生产的障碍，详细地在微生物范围内实施的工程途径策略，以及这些具有巨大应用的分子的商业准备。对于微生物细胞工厂生产的商业可行性，仍然存在一些需要解决的限制。尽管在成功生产这些脂肪酸衍生化学品方面取得了实质性进展，但需要做大量工作来提高效价。在本综述中，我们的目标是解决阻碍异源生产的障碍，详细地在微生物范围内实施的工程途径策略，以及这些具有巨大应用的分子的商业准备。