The performance of the alkaline fungal laccase PIE5 (pH 8.5) in the delignification and detoxification of alkali-pretreated corncob to produce bioethanol was evaluated and compared with that of the neutral counterpart (rLcc9, 6.5), with the acidic laccase rLacA (4.0) was used as an independent control. Treatment with the three laccases facilitated bioethanol production compared with their respective controls. The lignin contents of alkali-pretreated corncob reduced from 4.06%, 5.06%, and 7.80% to 3.44%, 3.95%, and 5.03%, after PIE5, rLcc9, and rLacA treatment, respectively. However, the performances of the laccases were in the order rLacA > rLcc9 > PIE5 in terms of decreasing total phenol concentration (0.18, 0.36, and 0.67 g/l), boosting ethanol concentration (8.02, 7.51, and 7.31 g/l), and volumetric ethanol productivity (1.34, 0.94, and 0.91 g/l hr), and shortening overall fermentation time. Our results would inform future attempts to improve laccases for ethanol production. Furthermore, based on our data and the fact that additional procedures, such as pH adjustment, are needed during neutral/alkaline fungal laccase treatment, we suggest acidic fungal laccases may be a better choice than neutral/alkaline fungal laccases in bioethanol production.

中文翻译：

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不同真菌漆酶对碱预处理玉米芯用于生物乙醇生产的脱木质素和解毒性能比较

评估了碱性真菌漆酶 PIE5 (pH 8.5) 在碱预处理玉米芯脱木素和解毒生产生物乙醇中的性能，并与中性漆酶 (rLcc9, 6.5) 进行了比较，酸性漆酶 rLacA (4.0) 为用作独立控件。与它们各自的对照相比，用三种漆酶处理促进了生物乙醇的产生。经过 PIE5、rLcc9 和 rLacA 处理后，碱预处理玉米芯的木质素含量分别从 4.06%、5.06% 和 7.80% 降低到 3.44%、3.95% 和 5.03%。然而，漆酶的性能顺序为rLacA＞1。rLcc9＞PIE5 在降低总苯酚浓度（0.18、0.36 和 0.67 g/l）、提高乙醇浓度（8.02、7.51 和 7.31 g/l）和体积乙醇生产率（1.34、0. 94 和 0.91 g/l hr)，并缩短了整体发酵时间。我们的结果将为未来改进漆酶用于乙醇生产的尝试提供信息。此外，根据我们的数据以及在中性/碱性真菌漆酶处理过程中需要额外的程序（例如 pH 调节）的事实，我们建议酸性真菌漆酶可能是生物乙醇生产中比中性/碱性真菌漆酶更好的选择。