Morphological mutants of Trichoderma reesei were isolated following chemical or insertional mutagenesis. The mutant strains were shown to have reduced viscosity under industrially relevant fermentation conditions and to have maintained high specific productivity of secreted protein. This allowed higher biomass concentration to be maintained during the production phase and, consequently, increased volumetric productivity of secreted protein. The causative mutations were traced to four individual genes (designated sfb3, ssb7, seb1, and mpg1). We showed that two of the morphological mutations could be combined in a single strain to further reduce viscosity and enable a 100% increase in volumetric productivity.

中文翻译：

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具有改进的工业发酵特性的里氏木霉的降低粘度突变体

经过化学或插入诱变分离出里氏木霉的形态突变体。突变菌株在工业相关发酵条件下显示出粘度降低，并保持了分泌蛋白的高比生产率。这使得在生产阶段能够保持较高的生物量浓度，从而提高了分泌蛋白的体积生产率。致病突变可追溯到四个单独的基因（称为 sfb3、ssb7、seb1 和 mpg1）。我们证明，两种形态突变可以组合在一个菌株中，以进一步降低粘度并使体积生产率提高 100%。