Background The Eclipse Farm 4G test coupled to the COMET device allows for automatic and easy screening of a broad range of antimicrobials in raw bovine milk. All results obtained with this system are available in real-time through a smartphone application (App) and in the Test4all cloud platform. Objective The objective of this study was to validate Eclipse Farm 4G & COMET for the screening of antimicrobials in raw bovine milk according to AOAC Performance Tested MethodSM procedures. Method The test is based on the inhibition of microbial growth of Geobacillus stearothermophilus in the presence of antimicrobials. When sample tubes are incubated in the absence of antibiotics, spores germinate and cells grow, changing the medium color from purple to yellow (negative result). The presence of antimicrobials in the milk sample hinders bacterial growth, preventing the tubes from changing color from purple to yellow (positive result). The COMET device automatically integrates all assay steps (incubation, time control, and results interpretation) and communications between the device, smartphone, and cloud. Results LODs and detection capabilities were confirmed to be at or below the European Union (EU) maximum residue limit (MRL) for most of the evaluated molecules representing the main families of antimicrobials (penicillins, cephalosporins, tetracyclines, sulfonamides, macrolides, and aminoglycosides). False-positive rates and the effect of potentially interfering substances showed sufficient selectivity/specificity. The Eclipse Farm 4G & COMET system was shown to be robust, consistent, and stable during shelf life. Conclusions The Eclipse Farm 4G & COMET system has been shown to be suitable for screening antibiotics in raw bovine milk. Highlights The assay is an automatic and easy system for the detection of a broad range of antibiotics in raw bovine milk consistent with EU MRLs and can provide results any time and anywhere through a smartphone App and a cloud platform.

中文翻译：

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Eclipse Farm 4G 和 COMET 用于检测生牛奶中抗生素的验证：AO​​AC 性能测试方法SM 022101

背景 Eclipse Farm 4G 测试与 COMET 设备相结合，可以自动轻松地筛选生牛奶中的各种抗菌剂。使用该系统获得的所有结果都可以通过智能手机应用程序 (App) 和 Test4all 云平台实时获得。目的 本研究的目的是验证 Eclipse Farm 4G 和 COMET 根据 AOAC 性能测试方法 SM 程序筛选生牛奶中的抗菌剂。方法 该测试基于在抗微生物剂存在下抑制嗜热脂肪地芽孢杆菌的微生物生长。当样品管在没有抗生素的情况下孵育时，孢子会发芽并生长，从而将培养基颜色从紫色变为黄色（阴性结果）。牛奶样品中存在的抗菌剂会阻碍细菌生长，防止试管颜色从紫色变为黄色（阳性结果）。COMET 设备自动集成所有分析步骤（孵育、时间控制和结果解释）以及设备、智能手机和云之间的通信。结果 确认代表主要抗菌药物家族（青霉素、头孢菌素、四环素、磺胺类、大环内酯类和氨基糖苷类）的大多数评估分子的 LOD 和检测能力等于或低于欧盟 (EU) 最大残留限量 (MRL) . 假阳性率和潜在干扰物质的影响显示出足够的选择性/特异性。Eclipse Farm 4G & COMET 系统在保质期内被证明是稳健、一致和稳定的。结论 Eclipse Farm 4G & COMET 系统已被证明适用于筛选生牛奶中的抗生素。亮点 该检测是一种自动且简单的系统，用于检测与欧盟 MRL 一致的生牛奶中的多种抗生素，并可通过智能手机应用程序和云平台随时随地提供结果。