ABSTRACT

Capsicum annuum is also known as chili which is one of the most important vegetable crops grown in the world. Breeding new varieties with heterosis could improve the quality of pepper, increase yield, growth potential, disease resistance, adaptability, and seed viability. To investigate the heterosis among three cross combinations of different parents, the mRNA-miRNA integrated analysis was performed. A total number of 22,659,009 to 36,423,818 clean data were generated from mRNA-seq with 81 libraries, and the unique mapped reads were from 35,495,567 (86.81%) to 46,466,622 (88.95%). The plant-hormone signal transduction pathway (40 genes) was detected with a higher DEG number. The SAUR32L, GID1, PYR1, EIN2. ERF1, PR1, JAR1-like, IAA from this pathway play a key role in plant development. From the miRNA-seq, the number of clean reads was ranging from 12,132,221 to 25,632,680. A total of 220 miRNAs were predicted in this study, and all of them were identified as novel miRNA. The top three candidate KEGG pathways of miRNA were ribosome signaling pathway (13 miRNAs), spliceosome pathway (13 miRNAs), and plant hormone signal transduction pathways (10 miRNAs). With the mRNA and miRNA integrated analysis, we found some key genes were regulated by some miRNAs. Among them, the scarecrow-like 6 protein can be up or down regulated by mir8, mir120, mir184, mir_214, mir125, and mir130. The function of Della protein was regulated by mir24, mir74, mir94, mir139, and mir190. This study contributes to understanding how heterosis regulates the traits, such as crop production, fruit weight, and fruit length.

摘要

辣椒又称辣椒，是世界上最重要的蔬菜作物之一。选育具有杂种优势的新品种可以提高辣椒的品质，增加产量、生长潜力、抗病性、适应性和种子活力。为了研究不同亲本的三个杂交组合之间的杂种优势，进行了mRNA-miRNA整合分析。使用 81 个文库从 mRNA-seq 生成了 22,659,009 到 36,423,818 条干净数据，唯一映射读取从 35,495,567 (86.81%) 到 46,466,622 (88.95%)。检测到植物激素信号转导途径（40 个基因）具有较高的 DEG 数。SAUR32L 、GID1、PYR1、EIN2。ERF1、PR1、JAR1 样、IAA从这个途径在植物发育中起关键作用。从 miRNA-seq 来看，干净读取的数量从 12,132,221 到 25,632,680 不等。本研究共预测出220个miRNA，均被鉴定为新型miRNA。miRNA 的前三位候选 KEGG 通路是核糖体信号通路（13 个 miRNA）、剪接体通路（13 个 miRNA）和植物激素信号转导通路（10 个 miRNA）。通过对mRNA和miRNA的综合分析，我们发现一些关键基因受到一些miRNA的调控。其中稻草人样6蛋白可通过mir8、mir120、mir184、mir_214、mir125、mir130上调或下调。Della蛋白的功能受mir24、mir74、mir94、mir139和mir190的调节。这项研究有助于了解杂种优势如何调节性状，如作物产量、果实重量、