Using nontoxic craft items and disposable lab consumables, we have developed nine modules to teach fundamental, hands-on microbiology lab skills safely at home. These "Crafty" teaching modules can be paired with virtual instruction and/or data collected by an instructor to replicate traditional microbiology lab exercises that characterize an unknown microbe. Materials and procedures used were carefully chosen to best mimic the texture of media, represent microbial diversity, assess aseptic technique, and produce analyzable data from results. Some protocols build upon and extend previously unpublished ideas, while others provide novel methods. The lab skills include proper personal protective equipment usage and basic biosafety, aseptic technique, microscopy and staining, streaking for isolation, spread plating, serial dilutions, filtering, disk diffusion method, and modeling an epidemic. Each protocol includes a student handout with background, links to videos of the methods performed with microbes, a rationale for the pairing of craft and consumable lab supplies along with technique used, a video or image demonstration of the "Crafty" technique when needed, postlab questions, and an instructor guide. This resource was developed for an undergraduate microbiology course, and each lab is aligned with learning outcomes within the American Society for Microbiology's undergraduate curriculum guidelines. This work would also be useful for outreach and K-12 educators. The development of microbiology lab skills by all students, regardless of economic or health status, will lead to a more scientifically minded society.

中文翻译：

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教授“狡猾的微生物学”：在家安全地教授微生物学实践技能。


使用无毒工艺品和一次性实验室耗材，我们开发了九个模块，可以在家安全地教授基本的、实用的微生物学实验室技能。这些“狡猾”的教学模块可以与虚拟教学和/或讲师收集的数据配合使用，以复制表征未知微生物的传统微生物学实验室练习。使用的材料和程序都经过精心挑选，以最好地模拟培养基的质地，代表微生物多样性，评估无菌技术，并从结果中产生可分析的数据。一些协议建立并扩展了以前未发表的想法，而另一些协议则提供了新颖的方法。实验室技能包括正确使用个人防护设备和基本生物安全、无菌技术、显微镜和染色、划线隔离、铺板、连续稀释、过滤、纸片扩散法和流行病建模。每个协议包括一份带有背景的学生讲义、微生物方法的视频链接、工艺和消耗性实验室用品以及所使用技术的配对原理、需要时“狡猾”技术的视频或图像演示、实验后问题和讲师指南。该资源是为本科微生物学课程开发的，每个实验室都符合美国微生物学会本科课程指南中的学习成果。这项工作对于外展和 K-12 教育工作者也很有用。所有学生，无论经济状况或健康状况如何，培养微生物学实验室技能，都将导致社会更加具有科学意识。