The insecticides of microbial origin may be promising biocontrol agents to control pests. In this study, a major metabolite was extracted and purified from Serratia marcescens KH-001 and identified as prodigiosin with a molecule weight of 324.2094, and our primary aim was to evaluate the effects of prodigiosin against Diaphorina citri Kuwayama (Hemiptera: Liviidae) in the laboratory and in the field. Toxicity of purified prodigiosin to nymphs increased with increasing temperature from 25 to 35°C. The laboratory experiment results observed inhibition effects of purified prodigiosin at LC50 on oviposition and egg hatch of D. citri at 30°C. Furthermore, adults that fed on citrus leaves treated with LC20 and LC50 solution of purified prodigiosin at 30°C excreted less honeydew (28–35%) compared with controls, suggesting moderate antifeedant activity of prodigiosin. The results of field experiment showed that 10% prodigiosin emulsifiable concentrate exhibited better control efficacy (70–100%) at three concentrations (100, 200, and 500 mg/liter) in July and August than in October. These data indicate that prodigiosin will be a highly effective compound for the control of D. citri depending on the prevailing temperature conditions and may become a commercially available product to be widely used in citrus orchards.

中文翻译：

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评估粘质沙雷氏菌 KH-001 中灵菌红素对亚洲柑橘木虱的生物潜力

微生物来源的杀虫剂可能是控制害虫的有前途的生物防治剂。在这项研究中，从粘质沙雷氏菌 KH-001 中提取和纯化了一种主要代谢物，并鉴定为灵菌红素，分子量为 324.2094，我们的主要目的是评估灵菌红素对柑橘红山毛虫（半翅目：Liviidae）的影响。实验室和现场。纯化的灵菌红苷对若虫的毒性随着温度从 25°C 升高到 35°C 而增加。实验室实验结果观察到纯化的灵菌红苷在 LC50 对 30°C 柑橘木霉产卵和卵孵化的抑制作用。此外，与对照组相比，以 30°C 纯化灵菌红素的 LC20 和 LC50 溶液处理过的柑橘叶为食的成虫排泄的蜜露更少（28-35%），表明灵菌红素具有中等拒食活性。田间试验结果表明，10%灵菌红素乳油在100、200、500 mg/L三个浓度下的防治效果（70-100%）在7月和8月均优于10月。这些数据表明，灵菌红素将是一种高效的化合物，可根据当时的温度条件控制柑橘红斑病菌，并可能成为广泛用于柑橘园的市售产品。