Background: There is growing evidence that polymorphisms in chemokine and chemokine receptor genes influence susceptibility to HIV infection and disease progression. However, not much is documented about the influence of these polymorphisms in HIV serodiscordant couples in Cameroon.

Objective: The objective of this study therefore was to determine the prevalence and the effect of the polymorphisms of CCR5-Δ32, CCR5 promoter 59029 A/G, CCR2-64I and SDF1-3′A gene in HIV serodiscordant couples in comparison to HIV negative seroconcordant and HIV positive seroconcordant couples in Yaoundé-Cameroon.

Methods: A total of 96 couples were recruited from five hospitals, of which 60 couples were HIV serodiscordant (test group), 18 HIV negative seroconcordant and 18 HIV positive seroconcordant couples were used as controls. Their genotypes for CCR5-Δ32, CCR5 promoter, CCR2 and SDF1 were analyzed using polymerase chain reaction (PCR) and restriction fragment length polymorphism.

Results: The allelic frequencies of these genes in the studied population were: 0%, 26.30%, 15.30% and 1.62% respectively for CCR5-Δ32, CCR5 promoter, CCR2 and SDF1. The frequency of the combination of CCR5 promoter and SDF1- (A/A+ G/G) wild-type genotype was higher in HIV-infected partners (82.92%) compared to uninfected partners (56.1%) in HIV serodiscordant couples (p= 0.0001). The combination of wild-type CCR2 and SDF1 genotypes (G/G + G/G) was higher among uninfected partners (80.48%) in HIV serodiscordant couples compared to the infected partners (60.97) (p= 0.005).

Conclusion: HIV negative partner protection against HIV/AIDS infection may be attributed to the combination of wild-type genotypes (G/G and G/G) of CCR2 and SDF1 genes in HIV serodiscordant couples.

背景：越来越多的证据表明，趋化因子和趋化因子受体基因的多态性会影响对 HIV 感染和疾病进展的易感性。然而，关于这些多态性对喀麦隆 HIV 血清不一致夫妇的影响的文献记载不多。

目的：因此，本研究的目的是确定与 HIV 阴性相比，HIV 血清不一致夫妇中 CCR5-Δ32、CCR5 启动子 59029 A/G、CCR2-64I 和 SDF1-3'A 基因多态性的流行率和影响雅温得-喀麦隆的血清一致和 HIV 阳性的血清一致夫妇。

方法：共招募来自5家医院的96对夫妻，其中HIV血清不一致60对（试验组），HIV阴性18对，HIV阳性18对作为对照。使用聚合酶链式反应 (PCR) 和限制性片段长度多态性分析其 CCR5-Δ32、CCR5 启动子、CCR2 和 SDF1 的基因型。

结果：研究人群中这些基因的等位基因频率分别为：CCR5-Δ32、CCR5启动子、CCR2和SDF1的等位基因频率分别为：0%、26.30%、15.30%和1.62%。在 HIV 血清不一致的夫妇中，CCR5 启动子和 SDF1- (A/A+ G/G) 野生型基因型组合的频率（82.92%）高于未感染的伴侣（56.1%）（p=0.0001 ）。与受感染的伴侣（60.97）相比，HIV 血清不一致夫妇中未感染伴侣（80.48%）的野生型 CCR2 和 SDF1 基因型（G/G + G/G）的组合更高（p = 0.005）。

结论： HIV 阴性伴侣对 HIV/AIDS 感染的保护可能归因于 HIV 血清不一致夫妇中 CCR2 和 SDF1 基因的野生型基因型（G/G 和 G/G）的组合。