Metabolic engineering reprograms cells to synthesize value-added products. In doing so, endogenous genes are altered and heterologous genes can be introduced to achieve the necessary enzymatic reactions. Dynamic regulation of metabolic flux is a powerful control scheme to alleviate and overcome the competing cellular objectives that arise from the introduction of these production pathways. This review explores dynamic regulation strategies that have demonstrated significant production benefits by targeting the metabolic node corresponding to a specific challenge. We summarize the stimulus-responsive control circuits employed in these strategies that determine the criterion for actuating a dynamic response and then examine the points of control that couple the stimulus-responsive circuit to a shift in metabolic flux. Expected final online publication date for the Annual Review of Chemical and Biomolecular Engineering, Volume 12 is June 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

中文翻译：

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代谢的动态控制。

代谢工程重新编程细胞以合成增值产品。这样，内源基因被改变并且异源基因可以被引入以实现必要的酶促反应。代谢通量的动态调节是缓解和克服因引入这些生产途径而引起的竞争性细胞目标的有力控制方案。这篇综述探讨了动态调节策略，这些策略通过针对与特定挑战相对应的代谢节点展示了显着的生产益处。我们总结了在这些策略中使用的刺激响应控制电路，这些电路确定了激活动态响应的标准，然后研究了将刺激响应电路耦合到代谢通量变化的控制点。