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Optobiochemistry: Genetically Encoded Control of Protein Activity by Light
Annual Review of Biochemistry ( IF 12.1 ) Pub Date : 2021-06-21 , DOI: 10.1146/annurev-biochem-072420-112431
Jihye Seong 1 , Michael Z Lin 2, 3, 4
Affiliation  

Optobiochemical control of protein activities allows the investigation of protein functions in living cells with high spatiotemporal resolution. Over the last two decades, numerous natural photosensory domains have been characterized and synthetic domains engineered and assembled into photoregulatory systems to control protein function with light. Here, we review the field of optobiochemistry, categorizing photosensory domains by chromophore, describing photoregulatory systems by mechanism of action, and discussing protein classes frequently investigated using optical methods. We also present examples of how spatial or temporal control of proteins in living cells has provided new insights not possible with traditional biochemical or cell biological techniques.

中文翻译:


光生物化学:光对蛋白质活性的基因编码控制

蛋白质活性的光生化控制允许以高时空分辨率研究活细胞中的蛋白质功能。在过去的二十年里,许多天然光感应结构域已经被表征,合成结构域被设计并组装到光调节系统中,以用光控制蛋白质功能。在这里,我们回顾了光生物化学领域,通过发色团对光感应域进行分类,通过作用机制描述光调节系统,并讨论使用光学方法经常研究的蛋白质类别。我们还提供了活细胞中蛋白质的空间或时间控制如何提供传统生化或细胞生物学技术无法提供的新见解的示例。

更新日期:2021-06-22
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