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Applications of Single-Cell DNA Sequencing
Annual Review of Genomics and Human Genetics ( IF 7.7 ) Pub Date : 2021-08-31 , DOI: 10.1146/annurev-genom-111320-090436
Gilad D Evrony 1 , Anjali Gupta Hinch 2 , Chongyuan Luo 3
Affiliation  

Over the past decade, genomic analyses of single cells—the fundamental units of life—have become possible. Single-cell DNA sequencing has shed light on biological questions that were previously inaccessible across diverse fields of research, including somatic mutagenesis, organismal development, genome function, and microbiology. Single-cell DNA sequencing also promises significant future biomedical and clinical impact, spanning oncology, fertility, and beyond. While single-cell approaches that profile RNA and protein have greatly expanded our understanding of cellular diversity, many fundamental questions in biology and important biomedical applications require analysis of the DNA of single cells. Here, we review the applications and biological questions for which single-cell DNA sequencing is uniquely suited or required. We include a discussion of the fields that will be impacted by single-cell DNA sequencing as the technology continues to advance.

中文翻译:



单细胞 DNA 测序的应用



在过去的十年中,单细胞(生命的基本单位)的基因组分析已成为可能。单细胞 DNA 测序揭示了以前在不同研究领域无法解决的生物学问题,包括体细胞突变、有机体发育、基因组功能和微生物学。单细胞 DNA 测序还有望在未来产生重大的生物医学和临床影响,涵盖肿瘤学、生育力等领域。虽然分析 RNA 和蛋白质的单细胞方法极大地扩展了我们对细胞多样性的理解,但生物学和重要生物医学应用中的许多基本问题都需要分析单细胞的 DNA。在这里,我们回顾了单细胞 DNA 测序特别适合或需要的应用和生物学问题。我们讨论了随着技术的不断进步,单细胞 DNA 测序将影响的领域。

更新日期:2021-09-01
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