The present study investigated the effects of Bacillus coagulans and yeast hydrolysate supplementation on growth performance, immune response and intestinal barrier function of weaned piglets. Twenty-four weaned piglets with an average body weight (BW) of 6.89 ± 0.15 kg were divided into four diets for 28 days. The treatments were basal diet (control), basal diet supplemented with antibiotic (20 mg/kg colistin sulphate and 40 mg/kg bacitracin zinc, AT), probiotics (400 mg/kg Bacillus coagulans ≥5 × 109 CFU/g, BC) or yeast hydrolysate (5000 mg/kg yeast hydrolysate, YH). Average daily gain (ADG) and average daily feed intake (ADFI) were improved by AT and YH diets (p < 0.05), while BC diet only increased ADG (p < 0.05). The complement 3 (C3), lysozyme (LZM) and tumour necrosis factor-α (TNF-α) concentrations in serum were increased in BC diet (p < 0.05). Feeding AT and YH caused the increase of jejunal villus height (p < 0.05), and a higher ratio of villus height/crypt depth was observed in AT, BC and YH groups (p < 0.05). The mRNA expression of zonula occludens-1 (ZO-1) in jejunal mucosa was up-regulated by AT, BC and YH diets (p < 0.05). Dietary AT, BC or YH inclusion decreased the interleukin-1β (IL-1β) concentration and TNF-α mRNA expression (p < 0.05), and YH supplementation even down-regulated toll-like receptor 4 (TLR4) and CD14 expressions (p < 0.05). In summary, the dietary administration of BC or YH both improves growth performance through promoting the intestinal barrier function, indicating both of them can serve as potential alternatives to antibiotics growth promoters for the piglet production.

中文翻译：

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日粮添加凝固芽孢杆菌和酵母水解物对断奶仔猪生长性能、免疫反应和肠道屏障功能的影响

本研究调查了添加凝结芽孢杆菌和酵母水解物对断奶仔猪生长性能、免疫反应和肠道屏障功能的影响。24 头平均体重 (BW) 为 6.89 ± 0.15 kg 的断奶仔猪被分成四种日粮，持续 28 天。处理为基础日粮（对照）、添加抗生素（20 mg/kg 硫酸粘菌素和 40 mg/kg 杆菌肽锌，AT）、益生菌（400 mg/kg凝结芽孢杆菌≥5 × 109 CFU/g，BC）的基础日粮或酵母水解物（5000 mg/kg 酵母水解物，YH）。AT 和 YH 日粮提高了平均日增重 (ADG) 和平均日采食量 (ADFI) ( p  < 0.05)，而 BC 日粮仅增加了 ADG ( p < 0.05)。BC 饮食中血清中的补体 3 (C3)、溶菌酶 (LZM) 和肿瘤坏死因子-α (TNF-α) 浓度增加 ( p  < 0.05)。饲喂AT和YH导致空肠绒毛高度增加（p  < 0.05），并且在AT、BC和YH组中观察到更高的绒毛高度/隐窝深度比率（p  < 0.05）。空肠黏膜封闭带- 1 (ZO - 1)的 mRNA 表达被 AT、BC 和 YH 饮食上调 ( p  < 0.05)。饮食中添加 AT、BC 或 YH 会降低白细胞介素-1β（IL-1β）浓度和TNF - α mRNA 表达（p <0.05），和YH补充甚至下调的收费-样受体4（TLR4）和CD14的表达（p  <0.05）。总之，日粮施用 BC 或 YH 均通过促进肠道屏障功能来提高生长性能，表明它们都可以作为抗生素生长促进剂的潜在替代品，用于仔猪生产。