Hyperproliferation of epidermal keratinocytes is a major histopathological feature of chronic Sarcoptes scabiei dermatitis. We investigated the immunohistochemical expression of several keratins in scabietic dermatitis in sheep and in the skin of healthy sheep, using a panel of commercially available anti-human antibodies for keratins. Keratins AE1/AE3 and 34BE12 were expressed in all epithelial structures in healthy skin. Keratin MNF116 was expressed in the stratum basale and in the three lowest layers of the stratum spinosum, in follicular epithelium and in apocrine glands. Keratin K5/6 expression was seen in the stratum basale, in the two lowest cell layers of the stratum spinosum, in the outer root sheath of hair follicles and in myoepithelial cells of apocrine glands. K14 expression was observed in the stratum basale, in locally extensive regions of the two lowest cell layers of the stratum spinosum, in the outer root sheath of hair follicles and in sebaceous glands. Immunolabelling of K19 antigen was confined to apocrine glands. In scabietic skin, immunolabelling of keratin 34BE12 was seen in all layers of hyperplastic stratum spinosum and stratum granulosum but was restricted to some locally extensive regions in hyperkeratotic and parakeratotic stratum corneum. Keratin MNF116 was widely labelled in all layers of hyperplastic stratum spinosum and stratum granulosum. There was expansive labelling of K5/6 keratin in all layers of hyperplastic stratum spinosum and in locally extensive regions of stratum granulosum, as well as in hyperkeratotic or parakeratotic stratum corneum. Expansive labelling of K14 keratin was detected in all layers of hyperplastic stratum spinosum and in the layers of the hyperplastic stratum granulosum. K5/6 and K14 keratins were also labelled in the inner root sheath of occasional hair follicles.

中文翻译：

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角蛋白在正常绵羊皮肤和疥螨引起的慢性皮炎中的免疫组织化学表达。

表皮角质形成细胞的过度增殖是慢性疥疮皮炎的主要组织病理学特征。我们使用一组市售的角蛋白抗人抗体，研究了几种角蛋白在绵羊和健康绵羊皮肤中的疥疮性皮炎中的免疫组织化学表达。角蛋白 AE1/AE3 和 34BE12 在健康皮肤的所有上皮结构中均有表达。角蛋白 MNF116 在基底层和棘层的三个最低层、滤泡上皮和大汗腺中表达。角蛋白 K5/6 表达见于基底层、棘层的两个最低细胞层、毛囊的外根鞘和大汗腺的肌上皮细胞。在基底层中观察到 K14 表达，在棘层的两个最低细胞层的局部广泛区域，在毛囊的外根鞘和皮脂腺中。K19 抗原的免疫标记仅限于顶泌腺。在疥疮皮肤中，在增生性棘层和颗粒层的所有层中都观察到角蛋白 34BE12 的免疫标记，但仅限于角化过度和角化不全的角质层中的一些局部广泛区域。角蛋白 MNF116 在增生的棘层和颗粒层的所有层中被广泛标记。在增生性棘层的所有层和颗粒层的局部广泛区域以及角化过度或角化不全的角质层中都有广泛的 K5/6 角蛋白标记。在增生的棘层的所有层和增生的颗粒层的层中检测到 K14 角蛋白的膨胀标记。K5/6 和 K14 角蛋白也标记在偶尔毛囊的内根鞘中。