Hyperproliferation of epidermal keratinocytes is a major histopathological feature of chronic Sarcoptes scabiei dermatitis. We investigated the immunohistochemical expression of several keratins in scabietic dermatitis in sheep and in the skin of healthy sheep, using a panel of commercially available anti-human antibodies for keratins. Keratins AE1/AE3 and 34BE12 were expressed in all epithelial structures in healthy skin. Keratin MNF116 was expressed in the stratum basale and in the three lowest layers of the stratum spinosum, in follicular epithelium and in apocrine glands. Keratin K5/6 expression was seen in the stratum basale, in the two lowest cell layers of the stratum spinosum, in the outer root sheath of hair follicles and in myoepithelial cells of apocrine glands. K14 expression was observed in the stratum basale, in locally extensive regions of the two lowest cell layers of the stratum spinosum, in the outer root sheath of hair follicles and in sebaceous glands. Immunolabelling of K19 antigen was confined to apocrine glands. In scabietic skin, immunolabelling of keratin 34BE12 was seen in all layers of hyperplastic stratum spinosum and stratum granulosum but was restricted to some locally extensive regions in hyperkeratotic and parakeratotic stratum corneum. Keratin MNF116 was widely labelled in all layers of hyperplastic stratum spinosum and stratum granulosum. There was expansive labelling of K5/6 keratin in all layers of hyperplastic stratum spinosum and in locally extensive regions of stratum granulosum, as well as in hyperkeratotic or parakeratotic stratum corneum. Expansive labelling of K14 keratin was detected in all layers of hyperplastic stratum spinosum and in the layers of the hyperplastic stratum granulosum. K5/6 and K14 keratins were also labelled in the inner root sheath of occasional hair follicles.

中文翻译：

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角蛋白在Sarcoptes scabiei导致的正常绵羊皮肤和慢性皮炎中的免疫组织化学表达。

表皮角质形成细胞的过度增殖是慢性Sarcoptes scabiei皮炎的主要组织病理学特征。我们使用了一组针对角蛋白的市售抗人抗体，研究了绵羊和健康绵羊皮肤中的sc疮性皮炎中几种角蛋白的免疫组织化学表达。角蛋白AE1 / AE3和34BE12在健康皮肤的所有上皮结构中表达。角蛋白MNF116在基底层和棘状骨层的最低的三个最低层，滤泡上皮和顶泌腺中表达。角蛋白K5 / 6表达见于基底层，棘状棘突层的两个最低细胞层，毛囊外根鞘和顶分泌腺肌上皮细胞中。在基底层中观察到K14表达，在多刺棘皮层的两个最低细胞层的局部广泛区域，毛囊的外根鞘和皮脂腺中。K19抗原的免疫标记仅限于顶泌腺。在角质层皮肤中，增生性刺突层和颗粒层的所有层均可见角蛋白34BE12的免疫标记，但仅限于角化过度角化和角化副角质层的一些局部广泛区域。角蛋白MNF116被广泛标记在增生性棘棘层和颗粒层的所有层中。在增生性棘状棘层的所有各层以及颗粒层的局部广泛区域以及角化过度或角化过度的角质层中，都有K5 / 6角蛋白的广泛标记。在增生性棘层的所有层和增生性颗粒层中都检测到K14角蛋白的扩展标记。K5 / 6和K14角蛋白也标记在偶发毛囊的内根鞘中。