Aim - To explore the role and possible mechanism of KNTC1 gene in the development of bladder cancer. Methods - The expression level of KNTC1 in bladder cancer tissues and adjacent tissues was detected by immuno-histochemistry. Mann-Whitney U, Spearman, and Kaplan-Meier analyses were used to analyze the correlation between KNTC1 expression level and various characteristics of bladder cancer cases. KNTC1 was knocked down by RNA interference to detect the proliferation, apoptosis, cell cycle, migration, and in vivo tumorigenesis of bladder cancer cells. Human apoptosis antibody array and Western blot were used to detect the expression level changes of related proteins after KNTC1 knockdown to explore the possible mechanism. Results - KNTC1 was highly expressed in bladder cancer tissues and was related to the pathological grade and overall survival rate of bladder cancer. Knockdown of KNTC1 can inhibit the proliferation, migration, and in vivo tumorigenesis of bladder cancer cells and promote apoptosis. KNTC1 knockdown changes the levels of many proteins in bladder cancer cells, including Caspase 3, Fas, p-Akt, CDK6, PIK3CA, and MAPK9. Conclusion - KNTC1 plays a crucial role in the development of bladder cancer, and our findings provide evidence for its use as a therapeutic target.

中文翻译：

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敲低KNTC1抑制人膀胱癌细胞的增殖，迁移和肿瘤发生，并诱导其凋亡。

目的-探讨KNTC1基因在膀胱癌发展中的作用及其可能的机制。方法-采用免疫组织化学方法检测膀胱癌组织及癌旁组织中KNTC1的表达水平。使用Mann-Whitney U，Spearman和Kaplan-Meier分析来分析KNTC1表达水平与膀胱癌病例的各种特征之间的相关性。RNA干扰敲低了KNTC1，以检测膀胱癌细胞的增殖，凋亡，细胞周期，迁移和体内肿瘤发生。用人类凋亡抗体阵列和Western blot检测KNTC1敲低后相关蛋白的表达水平变化，探讨其可能的机制。结果-KNTC1在膀胱癌组织中高表达，与膀胱癌的病理分级和总生存率有关。敲低KNTC1可以抑制膀胱癌细胞的增殖，迁移和体内肿瘤发生，并促进细胞凋亡。KNTC1敲低改变了膀胱癌细胞中许多蛋白质的水平，包括Caspase 3，Fas，p-Akt，CDK6，PIK3CA和MAPK9。结论-KNTC1在膀胱癌的发展中起着至关重要的作用，我们的发现为将其用作治疗靶标提供了证据。