European catfish (Silurus glanis) is a commercially important freshwater fish originating from Eastern Europe. The objective of this study was to examine the short-term storage of its eggs especially in relation to maintaining a low level of malformation in newly hatched fry. The eggs from freshly spawned individuals were stored separately in cell incubators at 17 and 22 °C under aerobic conditions. Changes in fertilization, hatching, and malformation were examined in eggs stored at 1, 3, 5, and 7 h post-stripping. The sperm used for fertilization showed very good motility rates (84-90%) and curvilinear velocity (110-125 μm/s), and straight-line velocity did not drop below 77 μm/s. For all females, a temperature of 17 °C was better than 22 °C for egg storage in vitro. Egg fertilization and total hatching decreased rapidly after 7 h storage at 17 °C. The storage time of eggs in vitro to fertilization should therefore not exceed 5 h at 17 °C. In all females, there was no difference in the total number of eggs hatching between 1 and 3 h of egg storage at 17 °C. The storage time of eggs did not correlate with the level of malformations of the fry. However, the level of hatching and malformations was clearly affected by the storage temperature of eggs when it was > 17 °C. Analysis showed that the storage time of eggs, temperature of storage, and individual females had a significant influence on fertilization and total hatching rates. Regression analysis confirmed a low correlation of fertilization and hatching rates with storage time of eggs.

中文翻译：

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欧洲temperatures鱼卵（Silurus glanis L.）卵在不同温度下的体外贮藏水平。

欧洲cat鱼（Silurus glanis）是源自东欧的重要商业淡水鱼。这项研究的目的是检查其鸡蛋的短期储存，特别是与保持新孵化的鱼苗的畸形水平保持较低水平有关。来自新鲜产卵个体的卵分别在有氧条件下分别在17和22°C的细胞培养箱中储存。检查在剥离后1、3、5和7小时储存的卵中受精，孵化和畸形的变化。用于受精的精子显示出极好的运动率（84-90％）和曲线速度（110-125μm/ s），直线速度没有下降到77μm/ s以下。对于所有雌性，体外保存卵的温度都为17°C高于22°C。在17°C下储存7 h后，卵子的受精率和总孵化率迅速下降。因此，卵在体外受精的储存时间在17°C下不应超过5 h。在所有雌性中，在17°C下储藏1至3 h的卵孵化总数没有差异。鸡蛋的储存时间与鱼苗的畸形程度无关。但是，当蛋的储存温度> 17°C时，孵化和畸形的程度显然会受到影响。分析表明，卵的贮藏时间，贮藏温度和雌性个体对受精率和总孵化率有显着影响。回归分析证实了受精和孵化率与卵的储存时间之间的相关性较低。因此，卵在体外受精的储存时间在17°C下不应超过5 h。在所有雌性中，在17°C下储藏1至3 h的卵孵化总数没有差异。鸡蛋的储存时间与鱼苗的畸形程度无关。但是，当蛋的储存温度> 17°C时，孵化和畸形的程度显然会受到影响。分析表明，卵的贮藏时间，贮藏温度和雌性个体对受精率和总孵化率有显着影响。回归分析证实了受精和孵化率与卵的储存时间之间的相关性较低。因此，卵在体外受精的储存时间在17°C下不应超过5 h。在所有雌性中，在17°C下储藏1至3 h的卵孵化总数没有差异。鸡蛋的储存时间与鱼苗的畸形程度无关。但是，当蛋的存储温度> 17°C时，孵化和畸形的程度显然会受到影响。分析表明，卵的贮藏时间，贮藏温度和雌性个体对受精率和总孵化率有显着影响。回归分析证实了受精和孵化率与卵的储存时间之间的相关性较低。鸡蛋的储存时间与鱼苗的畸形程度无关。但是，当蛋的储存温度> 17°C时，孵化和畸形的程度显然会受到影响。分析表明，卵的贮藏时间，贮藏温度和雌性个体对受精率和总孵化率有显着影响。回归分析证实了受精和孵化率与卵的储存时间之间的相关性较低。鸡蛋的储存时间与鱼苗的畸形程度无关。但是，当蛋的储存温度> 17°C时，孵化和畸形的程度显然会受到影响。分析表明，卵的贮藏时间，贮藏温度和雌性个体对受精率和总孵化率有显着影响。回归分析证实了受精和孵化率与卵的储存时间之间的相关性较低。