BACKGROUND Till to date whether adiponectin AdipoQ gene variation (rs 1501299) is associated with cardiovascular disease, still remains controversial. Therefore, we aimed to relate the SNP (rs1501299) of adiponectin gene and oxidative stress in context to CVD in Punjabi women of North West India. METHODS In the present case-control study menopausal women with CVD as cases (n=265) and menopausal women without CVD as controls (n=258) were recruited. Genotyping of rs1501299 single nucleotide polymorphism of adiponectin gene was carried out by RFLP-PCR analysis. Biochemical parameters were analyzed according to the standard procedures. RESULTS Distribution of homozygous TT genotype of normolipidemic (p=0.001) and hyperlipidemic (p=0.001) women with CVD was significantly more frequent as compared to women without CVD. rs1501299 T allele carriers with CVD also showed significant (p=0.001) higher frequency distribution as compared to women without CVD. Under recessive model of inheritance TT mutant type homozygotes conferred ~9 fold higher risk [p=0.001; OR= 9.60 (2.92-31.58)] towards CVD susceptibility for MDA>1.50; ~11 fold higher risk [p=0.007; OR= 11.11 (1.49-82.83)] towards CVD for LDL carbonyl protein>15.04 and ~9 fold higher risk [p=0.001; OR= 9.75 (2.30-41.22)] towards CVD susceptibility for SOD≤5.55. Under logistic regression analysis oxidative stress and TT genotype were significantly correlated with CVD. CONCLUSIONS Our study revealed significant association of AdipoQ (rs1501299) gene polymorphism and oxidative stress with cardiovascular disease in Punjabi women of North West India. However, additional studies are required to support these findings.

中文翻译：

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印度西北部旁遮普妇女群体中 AdipoQ 基因变异 (rs1501299) 和氧化应激与心血管疾病的关联。


背景迄今为止，脂联素AdipoQ基因变异(rs 1501299)是否与心血管疾病相关仍存在争议。因此，我们的目的是将脂联素基因的 SNP (rs1501299) 和氧化应激与印度西北部旁遮普妇女的 CVD 联系起来。方法 在本病例对照研究中，招募了患有 CVD 的绝经期妇女作为病例（n=265）和未患 CVD 的绝经期妇女作为对照（n=258）。采用RFLP-PCR分析对脂联素基因rs1501299单核苷酸多态性进行基因分型。根据标准程序分析生化参数。结果 与没有 CVD 的女性相比，患有 CVD 的正常血脂（p=0.001）和高脂血症（p=0.001）女性的纯合 TT 基因型分布明显更频繁。与没有 CVD 的女性相比，患有 CVD 的 rs1501299 T 等位基因携带者也表现出显着（p=0.001）更高的频率分布。在隐性遗传模型下，TT 突变型纯合子的风险高出约 9 倍 [p=0.001； OR= 9.60 (2.92-31.58)] 对 MDA>1.50 的 CVD 敏感性；风险高约 11 倍 [p=0.007； OR= 11.11 (1.49-82.83)] LDL 羰基蛋白 >15.04 的 CVD 风险高出约 9 倍 [p=0.001； OR= 9.75 (2.30-41.22)] 对 SOD≤5.55 的 CVD 敏感性。根据逻辑回归分析，氧化应激和TT基因型与CVD显着相关。结论 我们的研究揭示了印度西北部旁遮普妇女中 AdipoQ (rs1501299) 基因多态性和氧化应激与心血管疾病的显着相关性。然而，需要更多的研究来支持这些发现。