The clinicopathological implication and prospective molecular mechanisms of miRNA-145-5p in the metastasis of prostate cancer (PCa) stand unclear. Herein, it is found that miRNA-145-5p expression was remarkably reduced in 131 cases of metastatic PCa than 1371 cases of localised ones, as the standardised mean differences (SMD) was -1.26 and the area under the curve (AUC) was 0.86, based on miRNA-chip and miRNA-sequencing datasets. The potential targets of miRNA-145-5p in metastatic PCa (n = 414) was achieved from the intersection of miRNA-145-5p transfected metastatic PCa cell line data, differential expression of metastatic PCa upregulated genes and online prediction databases. TOP2A was screened as one of the target hub genes by PPI network analysis, which was adversely related to miRNA-145-5p expression in both metastatic PCa (r = -0.504) and primary PCa (r = -0.281). Gene-chip and RNA-sequencing datasets, as well as IHC performed on clinical PCa samples, showed consistent upregulated expression of TOP2A mRNA and protein in PCa compared with non-PCa. The expression of TOP2A mRNA was also significantly higher in metastatic than localised PCa with the SMD being 1.72 and the AUC of sROC being 0.91. In summary, miRNA-145-5p may participate in PCa metastasis by binding TOP2A and be useful as a biomarker for the detection of metastatic PCa.

中文翻译：

---

MiRNA-145-5p在前列腺癌转移中的表达及前瞻性分子机制。

miRNA-145-5p在前列腺癌（PCa）转移中的临床病理意义和前瞻性分子机制尚不清楚。在本文中，发现转移性PCa 131例中的miRNA-145-5p表达比局部转移的1371例显着降低，因为标准平均差（SMD）为-1.26，曲线下面积（AUC）为0.86。 ，基于miRNA芯片和miRNA测序数据集。从miRNA-145-5p转染的转移性PCa细胞系数据，转移性PCa上调基因的差异表达和在线预测数据库的相交中，可以确定转移性PCa中miRNA-145-5p的潜在目标（n = 414）。通过PPI网络分析将TOP2A筛选为目标中心基因之一，这与两个转移性PCa中的miRNA-145-5p表达均呈负相关（r = -0。504）和主PCa（r = -0.281）。基因芯片和RNA测序数据集，以及对临床PCa样品进行的IHC显示，与非PCa相比，PCa中TOP2A mRNA和蛋白的表达一致上调。TOP2A mRNA的表达在转移中也明显高于局部PCa，其SMD为1.72，sROC的AUC为0.91。总之，miRNA-145-5p可以通过结合TOP2A参与PCa转移，并可用作检测转移性PCa的生物标记。72，sROC的AUC为0.91。总之，miRNA-145-5p可能通过结合TOP2A参与PCa转移，并可用作检测转移性PCa的生物标记。72，sROC的AUC为0.91。总之，miRNA-145-5p可以通过结合TOP2A参与PCa转移，并可用作检测转移性PCa的生物标记。